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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Varlet, V. Smith, F. de Froidmont, S. Dominguez, A. Rinaldi, A. Augsburger, M. Mangin, P. Grabherr, S. |
| Description | Country affiliation: Switzerland Author Affiliation: Varlet V ( Toxicology and Forensic Chemistry Unit, University Center of Legal Medicine Lausanne-Geneva,Lausanne, Switzerland. vincent.varlet@chuv.ch) |
| Abstract | A novel approach to measure carbon dioxide (CO2) in gaseous samples, based on a precise and accurate quantification by (13)CO2 internal standard generated in situ is presented. The main goal of this study was to provide an innovative headspace-gas chromatography-mass spectrometry (HS-GC-MS) method applicable in the routine determination of CO2. The main drawback of the GC methods discussed in the literature for CO2 measurement is the lack of a specific internal standard necessary to perform quantification. CO2 measurement is still quantified by external calibration without taking into account analytical problems which can often occur considering gaseous samples. To avoid the manipulation of a stable isotope-labeled gas, we have chosen to generate in situ an internal labeled standard gas ((13)CO2) on the basis of the stoichiometric formation of CO2 by the reaction of hydrochloric acid (HCl) with sodium hydrogen carbonate (NaH(13)CO3). This method allows a precise measurement of CO2 concentration and was validated on various human postmortem gas samples in order to study its efficiency. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 784 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-06-19 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Aorta, Thoracic Chemistry Carbon Dioxide Analysis Forensic Pathology Methods Gas Chromatography-mass Spectrometry Heart Atria Pathology Cadaver Calibration Carbon Isotopes Humans Isotope Labeling Reference Standards Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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