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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Song, Haiyan Ni, Yongnian Kokot, Serge |
| Description | Country affiliation: China Author Affiliation: Song H ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330031, China.) |
| Abstract | Hydrogen peroxide is an important analyte in biochemical, industrial and environmental systems. Therefore, development of novel rapid and sensitive analytical methods is useful. In this work, a hemin-graphene nano-sheets (H-GNs)/gold nano-particles (AuNPs) electrochemical biosensor for the detection of hydrogen peroxide (H2O2) was researched and developed; it was constructed by consecutive, selective modification of the GCE electrode. Performance of the H-GNs/AuNPs/GCE was investigated by chronoamperometry, and AFM measurements suggested that the graphene flakes thickness was ~1.3 nm and that of H-GNs was ~1.8 nm, which ultimately indicated that each hemin layer was ~0.25 nm. This biosensor exhibited significantly better electrocatalytic activity for the reduction of hydrogen peroxide in comparison with the simpler AuNPs/GCE and H-GNs/GCE; it also displayed a linear response for the reduction of H2O2 in the range of 0.3 µM to 1.8 mM with a detection limit of 0.11µM (SN(-1)=3), high sensitivity of 2774.8 µA mM(-1) cm(-2), and a rapid response, which reached 95% of the steady state condition within 5s. In addition, the biosensor was unaffected by many interfering substances, and was stable over time. Thus, it was demonstrated that this biosensor was potentially suitable for H2O2 analysis in many types of sample. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 788 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-07-25 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Biosensing Techniques Methods Electrochemical Techniques Instrumentation Electrodes Hydrogen Peroxide Analysis Nanostructures Chemistry Catalysis Dielectric Spectroscopy Gold Graphite Limit Of Detection Metal Nanoparticles Time Factors Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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