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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Naccarato, Attilio Gionfriddo, Emanuela Sindona, Giovanni Tagarelli, Antonio |
| Description | Country affiliation: Italy Author Affiliation: Naccarato A ( Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P. Bucci Cubo 12/C, Arcavacata di Rende (CS), I-87030 Cosenza, Italy.); Gionfriddo E ( Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P. Bucci Cubo 12/C, Arcavacata di Rende (CS), I-87030 Cosenza, Italy.); Sindona G ( Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P. Bucci Cubo 12/C, Arcavacata di Rende (CS), I-87030 Cosenza, Italy.); Tagarelli A ( Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P. Bucci Cubo 12/C, Arcavacata di Rende (CS), I-87030 Cosenza, Italy. Electronic address: a.tagarelli@unical.it.) |
| Abstract | The work aims at developing a simple and rapid method for the quantification of dopamine (DA), serotonin (5-HT) and norepinephrine (NE) in human urine. The urinary levels of these biogenic amines can be correlated with several pathological conditions concerning heart disease, stress, neurological disorders and cancerous tumors. The proposed analytical approach is based on the use of solid phase microextraction (SPME) combined with gas chromatography-triple quadrupole mass spectrometry (GC-QqQ-MS) after a fast derivatization of both aliphatic amino and phenolic moieties by propyl chloroformate. The variables influencing the derivatization reaction were reliably optimized by the multivariate approach of 'Experimental design'. The optimal conditions were obtained by performing derivatization with 100µL of propyl chloroformate and 100µL of pyridine. The extraction ability of five commercially available SPME fibers was evaluated in univariate mode and the best results were obtained using the polyacrylate fiber. The variables affecting the efficiency of SPME analysis were again optimized by the multivariate approach of 'Experimental design' and, in particular, a central composite design (CCD) was applied. The optimal values were extraction in 45min at room temperature, desorption temperature at 300°C, no addition of NaCl. Assay of derivatized analytes was performed by using a gas chromatography-triple quadrupole mass spectrometry (GC-QqQ-MS) system in selected reaction monitoring (SRM) acquisition. An evaluation of all analytical parameters demonstrates that the developed method provides satisfactory results. Indeed, very good linearities were achieved in the tested calibration range with correlation coefficient values of 0.9995, 0.9999 and 0.9997 for DA, 5-HT and NE, respectively. Accuracies and RSDs calculated for between-run and tested at concentrations of 30, 200, and 800µg L(-1) were in the range from 92.8% to 103.0%, and from 0.67 to 4.5%, respectively. Finally, the LOD values obtained can be considered very good (0.587, 0.381 and 1.23µg L(-1) for DA, 5-HT and NE, respectively). |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 810 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-01-31 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Chromatography, Gas Methods Dopamine Urine Mass Spectrometry Norepinephrine Serotonin Solid Phase Microextraction Adult Instrumentation Chemistry Female Healthy Volunteers Humans Male Molecular Structure Young Adult Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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