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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Castillo, Rosario del P. Araya, Juan Troncoso, Eduardo Vinet, Silenne Freer, Juanita |
| Description | Author Affiliation: Castillo Rdel P ( Faculty of Pharmacy, University of Concepcion, Concepcion, Chile); Araya J ( Faculty of Pharmacy, University of Concepcion, Concepcion, Chile); Troncoso E ( Consorcio Bioenercel S.A, University of Concepcion, Concepcion, Chile.); Vinet S ( Biotechnology Center, University of Concepcion, Concepcion, Chile); Freer J ( Biotechnology Center, University of Concepcion, Concepcion, Chile) |
| Abstract | The distribution and chemical patterns of lignocellulosic components at microscopic scale and their effect on the simultaneous saccharification and fermentation process (SSF) in the production of bioethanol from Pinus radiata pulps were analyzed by the application of diverse microscopical techniques, including scanning electronic microscopy (SEM), confocal laser scanning microscopy (CLSM) and attenuated total reflectance (ATR) - Fourier transform infrared microspectroscopy. This last technique was accompanied with multivariate methods, including principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS) to evaluate the distribution patterns and to generate pure spectra of the lignocellulosic components of fibers. The results indicate that the information obtained by the techniques is complementary (ultrastructure, confocality and chemical characterization) and that the distribution of components affects the SSF yield, identifying lignin coalescence droplets as a characteristic factor to increase the SSF yield. Therefore, multivariate analysis of the infrared spectra enabled the in situ identification of the cellulose, lignin and lignin-carbohydrates arrangements. These techniques could be used to investigate the lignocellulosic components distribution and consequently their recalcitrance in many applications where minimal sample manipulation and microscale chemical information is required. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 866 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-03-25 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Carbohydrates Chemistry Lignin Microscopy, Confocal Pinus Metabolism Spectroscopy, Fourier Transform Infrared Ethanol Fermentation Least-squares Analysis Microscopy, Electron, Scanning Principal Component Analysis Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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