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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Minghui Yu, Zhibiao Liu, Daofeng Peng, Tao Liu, Kun Wang, Shuying Xiong, Yonghua Wei, Hua Xu, Hengyi Lai, Weihua |
| Description | Country affiliation: China Author Affiliation: Chen M ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.); Yu Z ( Department of Science and Technology, Nanchang University, Nanchang, China.); Liu D ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.); Peng T ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.); Liu K ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.); Wang S ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.); Xiong Y ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China); Wei H ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China); Xu H ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China); Lai W ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China. Electronic address: talktolaiwh@163.com.) |
| Abstract | Two patterns of signal amplification lateral flow immunoassay (LFIA), which used anti-mouse secondary antibody-linked gold nanoparticle (AuNP) for dual AuNP-LFIA were developed. Escherichia coli O157:H7 was selected as the model analyte. In the signal amplification direct LFIA method, anti-mouse secondary antibody-linked AuNP (anti-mouse-Ab-AuNP) was mixed with sample solution in an ELISA well, after which it was added to LFIA, which already contained anti-E. coli O157:H7 monoclonal antibody-AuNP (anti-E. coli O157:H7-mAb-AuNP) dispersed in the conjugate pad. Polyclonal antibody was the test line, and anti-mouse secondary antibody was the control line in nitrocellulose (NC) membrane. In the signal amplification indirect LFIA method, anti-mouse-Ab-AuNP was mixed with sample solution and anti-E. coli O157:H7-mAb-AuNP complex in ELISA well, creating a dual AuNP complex. This complex was added to LFIA, which had a polyclonal antibody as the test line and secondary antibody as the control line in NC membrane. The detection sensitivity of both LFIAs improved 100-fold and reached 1.14×10(3) CFU mL(-1). The 28 nm and 45 nm AuNPs were demonstrated to be the optimal dual AuNP pairs. Signal amplification LFIA was perfectly applied to the detection of milk samples with E. coli O157:H7 via naked eye observation. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 876 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-05-30 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Escherichia Coli Infections Microbiology Escherichia Coli O157 Isolation & Purification Gold Chemistry Immunoassay Instrumentation Metal Nanoparticles Milk Animals Colony Count, Microbial Equipment Design Food Microbiology Ultrastructure Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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