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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ertürk, Gizem Hedström, Martin Tümer, M. Askin Denizli, Adil Mattiasson, Bo |
| Description | Country affiliation: Turkey Author Affiliation: Ertürk G ( Department of Biotechnology, Lund University, Lund, Sweden); Hedström M ( Department of Biotechnology, Lund University, Lund, Sweden); Tümer MA ( Department of Biology, Hacettepe University, Ankara, Turkey.); Denizli A ( Department of Chemistry, Hacettepe University, Ankara, Turkey.); Mattiasson B ( Department of Biotechnology, Lund University, Lund, Sweden) |
| Abstract | Prostate specific antigen (PSA) is a valuable biomarker for early detection of prostate cancer, the third most common cancer in men. Ultrasensitive detection of PSA is crucial to screen the prostate cancer in an early stage and to detect the recurrence of the disease after treatment. In this report, microcontact-PSA imprinted (PSA-MIP) capacitive biosensor chip was developed for real-time, highly sensitive and selective detection of PSA. PSA-MIP electrodes were prepared in the presence of methacrylic acid (MAA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-linker via UV polymerization. Immobilized Anti-PSA antibodies on electrodes (Anti-PSA) for capacitance measurements were also prepared to compare the detection performances of both methods. The electrodes were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM) and cyclic voltammetry (CV) and real-time PSA detection was performed with standard PSA solutions in the concentration range of 10 fg mL(-1)-100 ng mL(-1). The detection limits were found as 8.0 × 10(-5) ng mL(-1) (16 × 10(-17) M) and 6.0 × 10(-4) ng mL(-1) (12 × 10(-16) M) for PSA-MIP and Anti-PSA electrodes, respectively. Selectivity studies were performed against HSA and IgG and selectivity coefficients were calculated. PSA detection was also carried out from diluted human serum samples and finally, reproducibility of the electrodes was tested. The results are promising and show that when the sensitivity of the capacitive system is combined with the selectivity and reproducibility of the microcontact-imprinting procedure, the resulting system might be used successfully for real-time detection of various analytes even in very low concentrations. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 891 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-03 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Biosensing Techniques Instrumentation Prostate-specific Antigen Blood Antibodies, Immobilized Chemistry Electric Capacitance Electrodes Equipment Design Humans Limit Of Detection Male Methacrylates Polymerization Analysis Prostatic Neoplasms Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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