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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Munir, Sundas Park, Soo-Young |
| Description | Author Affiliation: Munir S ( School of Applied Chemical Engineering, Department of Polymer Science & Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.); Park SY ( School of Applied Chemical Engineering, Department of Polymer Science & Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: psy@knu.ac.kr.) |
| Abstract | Sodium dodecyl sulphate (SDS) including ß-cyclodextrin (ß-CD) (ß-CDSDS) was used to detect cholesterol at the 4-cyano-4'-pentylbiphenyl (5CB)/aqueous interface in transmission electron microscopy (TEM) grid cells. The ß-CD acts as a host for SDS (guest). The guest SDS enclosed within the ß-CD cavity was replaced with cholesterol by injecting cholesterol solution into the TEM cell at concentrations greater than 3 µM. The replacement of SDS with cholesterol was confirmed by pH measurement and high performance liquid chromatography (HPLC). The SDS excluded from the ß-CD altered the planar orientation of the 5CB confined within the TEM grid cell to a homeotropic orientation. This planar-to-homeotropic transition was observed using a polarized optical microscope under crossed polarizers. This convenient TEM grid cell provides a new method for the selective detection of cholesterol without immobilization of the detecting receptors (enzyme, antibody, or aptamer) or the use of sophisticated instruments. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 893 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-17 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Biosensing Techniques Cholesterol Analysis Liquid Crystals Chemistry Sodium Dodecyl Sulfate Beta-cyclodextrins Chromatography, High Pressure Liquid Hydrogen-ion Concentration Microscopy, Electron, Transmission Water Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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