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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Karimpour, Masoumeh Surowiec, Izabella Wu, Junfang Gouveia-Figueira, Sandra Pinto, Rui Trygg, Johan Zivkovic, Angela M. Nording, Malin L. |
| Description | Country affiliation: Sweden Author Affiliation: Karimpour M ( Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden.); Surowiec I ( Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden.); Wu J ( Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden.); Gouveia-Figueira S ( Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden); Pinto R ( Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden); Trygg J ( Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden.); Zivkovic AM ( Department of Nutrition, University of California, Davis, One Shields Ave, CA 95616, USA.); Nording ML ( Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden. Electronic address: malin.nording@umu.se.) |
| Abstract | The study of postprandial metabolism is relevant for understanding metabolic diseases and characterizing personal responses to diet. We combined three analytical platforms - gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) - to validate a multi-platform approach for characterizing individual variation in the postprandial state. We analyzed the postprandial plasma metabolome by introducing, at three occasions, meal challenges on a usual diet, and 1.5 years later, on a modified background diet. The postprandial response was stable over time and largely independent of the background diet as revealed by all three analytical platforms. Coverage of the metabolome between NMR and GC-MS included more polar metabolites detectable only by NMR and more hydrophobic compounds detected by GC-MS. The variability across three separate testing occasions among the identified metabolites was in the range of 1.1-86% for GC-MS and 0.9-42% for NMR in the fasting state at baseline. For the LC-MS analysis, the coefficients of variation of the detected compounds in the fasting state at baseline were in the range of 2-97% for the positive and 4-69% for the negative mode. Multivariate analysis (MVA) of metabolites detected with GC-MS revealed that for both background diets, levels of postprandial amino acids and sugars increased whereas those of fatty acids decreased at 0.5 h after the meal was consumed, reflecting the expected response to the challenge meal. MVA of NMR data revealed increasing postprandial levels of amino acids and other organic acids together with decreasing levels of acetoacetate and 3-hydroxybutanoic acid, also independent of the background diet. Together these data show that the postprandial response to the same challenge meal was stable even though it was tested 1.5 years apart, and that it was largely independent of background diet. This work demonstrates the efficacy of a multi-platform metabolomics approach followed by multivariate and univariate data analysis for a broad-scale screen of the individual metabolome, particularly for studies using repeated measures to determine dietary response phenotype. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 908 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-02-18 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Blood Metabolomics Postprandial Period Chromatography, Liquid Gas Chromatography-mass Spectrometry Humans Magnetic Resonance Spectroscopy Pilot Projects Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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