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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Peng, Minzhi Cai, Yanna Fang, Xiefan Liu, Li |
| Description | Country affiliation: China Author Affiliation: Peng M ( Department of Genetics and Endocrinology, Guangzhou Women and Children's Medical Center, The Affiliated Hospital of Guangzhou Medical University, 9 Jinsui Road, Guangzhou, China.); Cai Y ( Department of Genetics and Endocrinology, Guangzhou Women and Children's Medical Center, The Affiliated Hospital of Guangzhou Medical University, 9 Jinsui Road, Guangzhou, China.); Fang X ( Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL, USA.); Liu L ( Department of Genetics and Endocrinology, Guangzhou Women and Children's Medical Center, The Affiliated Hospital of Guangzhou Medical University, 9 Jinsui Road, Guangzhou, China. Electronic address: liliuxia2013@gmail.com.) |
| Abstract | A novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been established to quantify metabolic intermediates, including lactate (Lac), pyruvate (Pyr), acetoacetate (ACAC) and 3-hydroxybutyrate (3-HB) in blood. Samples were deproteinized with methanol-acetonitrile solution, and analytes were separated on an adamantyl group-bonded reverse phase column and detected in multiple reaction monitoring mode. Total analysis time was 4 min per sample. Method validation results displayed that limits of quantification were 10.0 µmol L for Lac and Pyr, and 5.0 µmol L for ACAC and 3-HB. The within- and between-run coefficients of variation were in the range of 1.2-6.4% for all analytes. The recoveries were ranged from 95.6 to 111.5%. The reference values of analytes were determined for the pediatric population. Duo to instability of Lac, Pyr and ACAC in vitro, a comprehensive stability assay was performed to determine optimal conditions for sample collection, pretreatment and storage. Results showed that precipitation of protein in blood at bedside combined with low storage temperature could effectively preserve the integrity of Lac, Pyr and 3-HB, but the precipitated protein accelerated degradation of ACAC. Isolation of supernatant fluid slowed degradation of ACAC. Supernatant samples could store at -20 °C for 10 days. The use of plasma or serum to determine these intermediates was not recommended. In this study, 450 samples from patients were analyzed, and 7 patients were diagnosed as congenital lactic acidosis. With the advantages of rapid, accurate and reliable, this method is very suitable for congenital lactic acidosis screening and researches related to energy metabolism. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Journal | Analytica Chimica Acta |
| Volume Number | 942 |
| e-ISSN | 18734324 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-10-26 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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