Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Tech Science Press |
|---|---|
| Author | Liu, Yaxue Ke, Yuwei Zhou, Yao Zhao, Yaqi Ma, Haoli Jin, Xiaoqing Li, Gang |
| Abstract | Quantitative real-time PCR (qRT-PCR) is widely used for investigating gene expression patterns and has many advantages, including its high sensitivity, fidelity, and specificity. Selecting a satisfactory internal reference gene is crucial for obtaining precise gene expression results in qRT-PCR analyses. In this study, the transcriptomic data of 2 potato varieties were screened for housekeeping genes with stable expression patterns. A total of 77 putative genes were selected, which were highly and stably expressed. Then, qRT-PCR analyses were performed to examine the expression levels of these 77 candidate reference genes in various potato tissues, including leaves, flowers, stolons, and tubers. Gene expression was represented by analyzing the Ct values at given threshold. Through geNorm and NormFinder program analyses, 10 candidate genes with the most stable expression patterns were obtained, including RPL19 , RPS15 , RPS9 , EF1α , TrxP1 , RPS8 , NTF , CAM , AACM , and RPS28 . Moreover, through the comprehensive analyses of 4 statistical algorithms (i.e., geNorm, NormFinder, BestKeeper, and RefFinder), results indicated that the most appropriate internal reference genes were RPL19 and EF1α . The obtained stable reference genes will contribute to future qRT-PCR analyses on potato tissue-related gene expression. |
| Related Links | https://www.techscience.com/phyton/v89n2/38675 |
| Starting Page | 329 |
| Ending Page | 344 |
| ISSN | 00319457 |
| DOI | 10.32604/phyton.2020.08874 |
| Issue Number | 2 |
| Journal | Phyton-International Journal of Experimental Botany (PHYTON) |
| Volume Number | 89 |
| e-ISSN | 18515657 |
| Language | English |
| Publisher Date | 2020-04-22 |
| Access Restriction | Open |
| Subject Keyword | RefFinder geNorm qRT-PCR potato tissues reference genes NormFinder BestKeeper |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|