NDLI: Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana)

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Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana)

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Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana)

Content Provider	Taylor & Francis Online
Author	Liu, Daicheng Han, Xiangning
Abstract	In this reported study, a novel high-performance thin-layer chromatography (HPTLC) method was developed for the detection and quantification of the toxic substance di(2-ethylhexyl) adipate (DEHA) in Antarctic krill. This procedure was based on the extraction of DEHA by ultrasonic solvent extraction with anhydrous ethanol, silica-gel column chromatographic separation, HPTLC detection and quantification using petroleum ether/ethyl acetate/ acetone/glacial acetic acid (29:1:0.5:2d*, v/v/v/v) as the developing solvent and bromine thymol blue solution as the chromogenic agent. The content of DEHA in freeze-dried Antarctic krill was found to be ca. 0.63 ± 0.05 mg/g. The structure of DEHA in the Antarctic krill was subsequently determined by gas chromatography–mass spectrometry (GC-MS) and infrared chromatography, which verified the presence of this compound in the krill. The HPTLC method exhibited excellent accuracy, with a recovery of 97.1–101.6% and good precision with a relative standard deviation of 2.47–4.90%. The DEHA in Antarctic krill oil was extracted by n-hexane and detected using the same method described above, which verified that DEHA was also present in krill oil at a concentration of ca. 2.16 ± 0.08 mg/g. The presence of DEHA in kill oil is very concerning because of its demonstrated harmful ecotoxicity, and since Antarctic krill is the key link in the food chain in the Antarctic coastal marine ecosystem. The adverse effects of DEHA on Antarctic krill and the source of DEHA will be explored in future research.
File Format	PDF HTM / HTML
ISSN	17518369
DOI	10.1080/17518369.2018.1457395
Journal	Polar Research
Volume Number	37
Issue Number	1
Language	English
Publisher	Routledge
Publisher Date	2018-05-09
Access Restriction	Open
Subject Keyword	Toxicity High-performance thin-layer chromatography (HPTLC) Ecotoxins Plastic particles Marine pollution Krill oil IR: infrared chromatography SD: standard deviation FTIR: Fourier-transform infrared spectroscopy HPTLC: high performance thin-layer chromatography DEHA: di(2-ethylhexyl) adipate TLC: thin-layer chromatography GC-MS: gas chromatography-mass spectrometry AU: absorbance unit HPLC: high-performance liquid chromatography RSD: relative standard deviation 2d: two drops
Content Type	Text
Resource Type	Article
Subject	1900/1901 Environmental Chemistry Environmental Science Oceanography

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