NDLI: Microfluidic platform for microbial spore germination studies in multiple growth conditions

Please wait, while we are loading the content...

Mosquito Tissue Ultrastructure-Expansion Microscopy (MoTissU-ExM) enables ultrastructural and anatomical analysis of malaria parasites and their mosquito

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Gap-repair recombineering for efficient retrieval of large DNA fragments from BAC clones and manipulation of large high-copy number plasmids

Advances in medical imaging techniques

Scalable isolation of surface-engineered extracellular vesicles and separation of free proteins via tangential flow filtration and size exclusion chromatography (TFF-SEC)

An end-to-end workflow to study newly synthesized mRNA following rapid protein depletion in Saccharomyces cerevisiae

Confirming size-exclusion chromatography as a clinically relevant extracellular vesicles separation method from 1mL plasma through a comprehensive comparison of methods

Magnetic procedure for sentinel lymph node detection and evaluation of metastases: design and rationale of the Lowmag trial

Mini-MEndR: a miniaturized 96-well predictive assay to evaluate muscle stem cell-mediated repair

Artificial intelligence for omics data analysis

Biomaterials and scaffolds for tissue engineering and regenerative medicine

Plant genome editing to achieve food and nutrient security

Empowering scientific progress: the vital role of open-access, peer-reviewed methods and protocols

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Content Provider	Springer Nature : BioMed Central
Author	Bernier, Léa S. Estoppey, Aislinn Bindschedler, Saskia Stan, Guy-Bart Junier, Pilar Stanley, Claire E.
Abstract	Background Spores are highly resistant dormant cells, adapted for survival and dispersal, that can withstand unfavourable environmental conditions for extended periods of time and later reactivate. Understanding the germination process of microbial spores is important in numerous areas including agriculture, food safety and health, and other sectors of biotechnology. Microfluidics combined with high-resolution microscopy allows to study spore germination at the single-cell level, revealing behaviours that would be hidden in standard population-level studies. Methods Here, we present a microfluidic platform – the so-called four-conditions microfluidic chemostat (4CMC) – for germination studies where spores are confined to monolayers inside microchambers, allowing the testing of four growth conditions in parallel. This platform can be used with multiple species, including non-model organisms, and is compatible with existing image analysis software. Results In this study, we focused on three soil dwellers, two bacteria and one fungus, and revealed new insights into their germination. We studied endospores of the model bacterium Bacillus subtilis and demonstrated a correlation between spore density and germination in rich media. We then investigated the germination of the obligate-oxalotrophic environmental bacterium Ammoniphilus oxalaticus in a concentration gradient of potassium oxalate, showing that lower concentrations result in more spores germinating compared to higher concentrations. We also used this microfluidic platform to study the soil beneficial filamentous fungus Trichoderma rossicum, showing for the first time that the size of the spores and hyphae increase in response to increased nutrient availability, while germination times remain the same. Discussion Our platform allows to better understand microbial behaviour at the single-cell level, under a variety of controlled conditions. While we used it to decipher the responsiveness of soil dwellers’ spores, it would also be suitable for other spores from bacteria or filamentous fungi, but also vegetative cells and yeast, and even microbial communities.
Related Links	https://bmcmethods.biomedcentral.com/counter/pdf/10.1186/s44330-024-00012-5.pdf
Ending Page	17
Page Count	17
Starting Page	1
File Format	HTM / HTML
ISSN	30048729
DOI	10.1186/s44330-024-00012-5
Journal	BMC Methods
Issue Number	1
Volume Number	1
Language	English
Publisher	BioMed Central
Publisher Date	2024-10-03
Access Restriction	Open
Subject Keyword	Biomedicine Medicine Public Health Life Sciences Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Artificial intelligence for omics data analysis

Advances in medical imaging techniques

Biomaterials and scaffolds for tissue engineering and regenerative medicine

BMC Methods

Plant genome editing to achieve food and nutrient security

At-line determining spore germination of Penicillium chrysogenum bioprocesses in complex media

Factors affecting spore germination in algae — review

Empowering scientific progress: the vital role of open-access, peer-reviewed methods and protocols

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Similar Documents

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Artificial intelligence for omics data analysis

Advances in medical imaging techniques

Biomaterials and scaffolds for tissue engineering and regenerative medicine

BMC Methods

Plant genome editing to achieve food and nutrient security

At-line determining spore germination of Penicillium chrysogenum bioprocesses in complex media

Factors affecting spore germination in algae — review

Empowering scientific progress: the vital role of open-access, peer-reviewed methods and protocols

Microfluidic platform for microbial spore germination studies in multiple growth conditions