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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Kfoury, Maria Hazzaz, Reda El Sanson, Claire Durand, Felix Blanc Michels, Judith Blameble, Emeline Colomba Tang, Roseline Le Formal, Audrey Lecerf, Elodie Gouy, Sebastien Maulard, Amandine Pautier, Patricia Rouleau, Etienne Leary, Alexandra |
| Abstract | Genomic testing is crucial for the management of ovarian cancer. DNA from biopsies at diagnostic laparoscopies or interval debulking surgery after neoadjuvant chemotherapy, has a high failure rate. At relapse, biopsies may not be feasible. The aim of our study was to evaluate the feasibility and usefulness of measuring genomic instability score (GIS) on cell-free DNA (cfDNA) from ascites.Patients enrolled in a prospective study (NCT03010124) consented to analysis of biological samples. CfDNA was extracted from 1 to 4 ml of double-centrifuged fresh ascites. Targeted Next-generation sequencing (NGS) including TP53 mutation (TP53m) was performed on cfDNA to confirm the presence of tumor cfDNA. Single Nucleotide Polymorphism Array estimating somatic copy number alterations (SCNA) was performed to calculate GIS for Homologous-Recombination deficiency (HRD).Twenty nine ascites were collected from 20 patients with suspected or confirmed OC. 93% (27/29) samples had detectable cfDNA (median 1120 ng [24-5732]) even when obtained during chemotherapy. A deleterious mutation was identified in 100%, with high allelic frequencies (median 60% [3.3–87%]), confirming that cfDNA was tumoral. SCNA analyses on 17 patients showed 11 high GIS, and 6 low GIS. 4 patients with confirmed BRCA mutation had a high GIS on ascites. When available from the same patient, SCNA profiles on ascites and tumor were superimposable.Ascites is frequent at diagnosis and relapse and yields large amounts of tumoral cfDNA. SCNA analysis on ascitic cfDNA is feasible and can detect the same HRD scar as tumor testing. Ascites could provide an alternative to tumor sampling for HRD and BRCA testing. |
| Related Links | https://biomarkerres.biomedcentral.com/counter/pdf/10.1186/s40364-023-00533-1.pdf |
| Ending Page | 4 |
| Page Count | 4 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 20507771 |
| DOI | 10.1186/s40364-023-00533-1 |
| Journal | Biomarker Research |
| Issue Number | 1 |
| Volume Number | 11 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2023-10-20 |
| Access Restriction | Open |
| Subject Keyword | Biomedicine Cancer Research Circulating Tumor DNA Ovarian cancer Homologous recombination deficiency Genomic instability score Ascites |
| Content Type | Text |
| Resource Type | Correspondence |
| Subject | Biochemistry (medical) Molecular Medicine Clinical Biochemistry |
| Journal Impact Factor | 9.5/2023 |
| 5-Year Journal Impact Factor | 9.4/2023 |
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