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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Xu, Guowei Xu, Shouxing Shi, Xijuan Shen, Chaochao Hao, Junhong Yan, Minhao Zhang, Dajun Zhu, Zixiang Zhang, Keshan Zheng, Haixue Liu, Xiangtao |
| Abstract | Seneca Valley virus (SVV) is a non-encapsulated single-stranded positive-strand RNA virus whose transmission routes have not yet been fully elucidated. Exosomes have been implicated in the intercellular transport of a variety of materials, such as proteins, RNA, and liposomes. However, whether exosomes can mediate SVV intercellular transmission remains unknown. In this study, we extracted exosomes from SVV-infected IBRS-2 cells to investigate intercellular transmission. Our results suggest that the intercellular transmission of SVV is mediated by exosomes. The results of co-localization and RT-qPCR studies showed that exosomes harbor SVV and enable the virus to proliferate in both susceptible and non-susceptible cells. Furthermore, the replication of SVV was inhibited when IBRS-2 cells were treated with interfering RNA Rab27a and exosome inhibitor GW4869. Finally, neutralization experiments were performed to further verify whether the virus was encapsulated by the exosomes that mediated transmission between cells. It was found that exosome-mediated intercellular transmission was not blocked by SVV-specific neutralizing antibodies. This study reveals a new transmission route of SVV and provides clear evidence regarding the pathogenesis of SVV, information which can also be useful for identifying therapeutic interventions. |
| Related Links | https://veterinaryresearch.biomedcentral.com/counter/pdf/10.1186/s13567-020-00812-x.pdf |
| Ending Page | 10 |
| Page Count | 10 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 12979716 |
| DOI | 10.1186/s13567-020-00812-x |
| Journal | Veterinary Research |
| Issue Number | 1 |
| Volume Number | 51 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2020-07-16 |
| Access Restriction | Open |
| Subject Keyword | Veterinary Medicine Veterinary Science Virology Microbiology Immunology Exosomes Infection Seneca valley virus Immune escape Intercellular transmission Veterinary Medicine/Veterinary Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary Medicine |
| Journal Impact Factor | 3.7/2023 |
| 5-Year Journal Impact Factor | 3.8/2023 |
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