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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Amjadi, Farhad Beheshti, Rahim Nasimi, Fatemeh Sokouti Hassani, Ayla Shirazi, Reza Tamadon, Amin Rahbarghazi, Reza Mahdipour, Mahdi |
| Abstract | Objective: Recently, the decellularization technique is introduced as one of the tissue engineering procedures for the treatment of various deficiencies. Here, we aimed to assess the dynamic activity of CCs and HUVECs within decellularized bovine ovarian tissue transplanted subcutaneously in rats. Ovarian tissue was decellularized using a cocktail consisting of different chemicals, and the efficiency of decellularization was assessed using hematoxylin-eosin and DAPI staining. The cell survival was evaluated using an LDH leakage assay. Thereafter, decellularized samples were recellularized using HUVECs and CCs, encapsulated inside alginate (1.2%)-gelatin, (1%) hydrogel, and transplanted subcutaneously to rats. The existence of CD31- and estrogen-positive cells was assessed using immunohistochemistry staining. Results: Bright-field imaging and DAPI staining revealed the lack of nuclei with naive matrix structure in ovarian tissue subjected to decellularization protocol. SEM imaging revealed a normal matrix in decellularized ovaries. LDH assay showed a lack of cytotoxicity for CCs after 7-days compared to the control group. Immunohistochemistry staining showed both CD31- and estrogen-positive cells in CCs + HUVECs compared to the CCs group. CD31 cells appeared with flattened morphology aligned with matrix fibers. The existence of estrogen and CD31 positive cells showed the efficiency of decellularized ovarian tissue to restore cellular function and activity. |
| Related Links | https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/s13104-022-06233-7.pdf |
| Ending Page | 8 |
| Page Count | 8 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 17560500 |
| DOI | 10.1186/s13104-022-06233-7 |
| Journal | BMC Research Notes |
| Issue Number | 1 |
| Volume Number | 15 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2022-11-08 |
| Access Restriction | Open |
| Subject Keyword | Biomedicine Medicine Public Health Life Sciences Bovine ovary Decellularization Recellularization Endothelial cells Cumulus cells Medicine/Public Health |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology Medicine |
| Journal Impact Factor | 1.6/2023 |
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