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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Hori, Asuka Ogata-Kawata, Hiroko Sasaki, Aiko Takahashi, Ken Taniguchi, Kosuke Migita, Ohsuke Kawashima, Akihiro Okamoto, Aikou Sekizawa, Akihiko Sago, Haruhiko Takada, Fumio Nakabayashi, Kazuhiko Hata, Kenichiro |
| Abstract | Objective We aimed to simplify our fetal RHD genotyping protocol by changing the method to attach Illumina’s sequencing adaptors to PCR products from the ligation-based method to a PCR-based method, and to improve its reliability and robustness by introducing unique molecular indexes, which allow us to count the numbers of DNA fragments used as PCR templates and to minimize the effects of PCR and sequencing errors. Results Both of the newly established protocols reduced time and cost compared with our conventional protocol. Removal of PCR duplicates using UMIs reduced the frequencies of erroneously mapped sequences reads likely generated by PCR and sequencing errors. The modified protocols will help us facilitate implementing fetal RHD genotyping for East Asian populations into clinical practice. |
| Related Links | https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/s13104-021-05793-4.pdf |
| Ending Page | 7 |
| Page Count | 7 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 17560500 |
| DOI | 10.1186/s13104-021-05793-4 |
| Journal | BMC Research Notes |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2021-09-26 |
| Access Restriction | Open |
| Subject Keyword | Biomedicine Medicine Public Health Life Sciences RHD Cell-free DNA (cfDNA) Non-invasive prenatal testing (NIPT) Amplicon sequencing Unique molecular identifier (UMI) Medicine/Public Health |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology Medicine |
| Journal Impact Factor | 1.6/2023 |
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