NDLI: Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803

Content Provider	Springer Nature : BioMed Central
Author	Xie, Hao Kjellström, Jarl Lindblad, Peter
Abstract	Background Cyanobacteria are emerging as green cell factories for sustainable biofuel and chemical production, due to their photosynthetic ability to use solar energy, carbon dioxide and water in a direct process. The model cyanobacterial strain Synechocystis sp. PCC 6803 has been engineered for the isobutanol and 3-methyl-1-butanol production by introducing a synthetic 2-keto acid pathway. However, the achieved productions still remained low. In the present study, diverse metabolic engineering strategies were implemented in Synechocystis sp. PCC 6803 for further enhanced photosynthetic isobutanol and 3-methyl-1-butanol production. Results Long-term cultivation was performed on two selected strains resulting in maximum cumulative isobutanol and 3-methyl-1-butanol titers of 1247 mg L−1 and 389 mg L−1, on day 58 and day 48, respectively. Novel Synechocystis strain integrated with a native 2-keto acid pathway was generated and showed a production of 98 mg isobutanol L−1 in short-term screening experiments. Enhanced isobutanol and 3-methyl-1-butanol production was observed when increasing the kivdS286T copy number from three to four. Isobutanol and 3-methyl-1-butanol production was effectively improved when overexpressing selected genes of the central carbon metabolism. Identified genes are potential metabolic engineering targets to further enhance productivity of pyruvate-derived bioproducts in cyanobacteria. Conclusions Enhanced isobutanol and 3-methyl-1-butanol production was successfully achieved in Synechocystis sp. PCC 6803 strains through diverse metabolic engineering strategies. The maximum cumulative isobutanol and 3-methyl-1-butanol titers, 1247 mg L−1 and 389 mg L−1, respectively, represent the current highest value reported. The significantly enhanced isobutanol and 3-methyl-1-butanol production in this study further pave the way for an industrial application of photosynthetic cyanobacteria-based biofuel and chemical synthesis from CO2.
Related Links	https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-023-02385-1.pdf
Ending Page	17
Page Count	17
Starting Page	1
File Format	HTM / HTML
ISSN	27313654
DOI	10.1186/s13068-023-02385-1
Journal	Biotechnology for Biofuels and Bioproducts
Issue Number	1
Volume Number	16
Language	English
Publisher	BioMed Central
Publisher Date	2023-09-09
Access Restriction	Open
Subject Keyword	Biotechnology Plant Breeding Environmental Engineering Renewable and Green Energy Microbiology Synechocystis sp. PCC 6803 2-Keto acid pathway Isobutanol 3-Methyl-1-butanol Cyanobacteria Metabolic engineering Solar fuel Plant Breeding/Biotechnology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Energy Management, Monitoring, Policy and Law Renewable Energy, Sustainability and the Environment Applied Microbiology and Biotechnology Biotechnology
Journal Impact Factor	6.1/2023
5-Year Journal Impact Factor	6/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Butanol tolerance regulated by a two-component response regulator Slr1037 in photosynthetic Synechocystis sp. PCC 6803

Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution

Effects of global transcription factor NtcA on photosynthetic production of ethylene in recombinant Synechocystis sp. PCC 6803

Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803

Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystissp. PCC 6803

Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803

Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803

Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria

Improving productivity of citramalate from CO2 by Synechocystis sp. PCC 6803 through design of experiment

Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803

Similar Documents

Butanol tolerance regulated by a two-component response regulator Slr1037 in photosynthetic Synechocystis sp. PCC 6803

Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution

Effects of global transcription factor NtcA on photosynthetic production of ethylene in recombinant Synechocystis sp. PCC 6803

Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803

Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystissp. PCC 6803

Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803

Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803

Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria

Improving productivity of citramalate from CO2 by Synechocystis sp. PCC 6803 through design of experiment

Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803