NDLI: From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae

Content Provider	Springer Nature : BioMed Central
Author	Ishii, Jun Okazaki, Fumiyoshi Djohan, Apridah Cameliawati Hara, Kiyotaka Y. Asai-Nakashima, Nanami Teramura, Hiroshi Andriani, Ade Tominaga, Masahiro Wakai, Satoshi Kahar, Prihardi Yopi Prasetya, Bambang Ogino, Chiaki Kondo, Akihiko
Abstract	Background Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays β-mannanase and β-mannosidase; this strain is expected to facilitate ethanol fermentation using mannan as a biomass source. Results Parental yeast S. cerevisiae assimilated mannose and glucose as monomeric sugars, producing ethanol from mannose. We constructed yeast strains that express tethered β-mannanase and β-mannosidase; co-display of the two enzymes on the cell surface was confirmed by immunofluorescence staining and enzyme activity assays. The constructed yeast cells successfully hydrolyzed 1,4-β-d-mannan and produced ethanol by assimilating the resulting mannose without external addition of enzymes. Furthermore, the constructed strain produced ethanol from 1,4-β-d-mannan continually during the third batch of repeated fermentation. Additionally, the constructed strain produced ethanol from ivory nut mannan; ethanol yield was improved by NaOH pretreatment of the substrate. Conclusions We successfully displayed β-mannanase and β-mannosidase on the yeast cell surface. Our results clearly demonstrate the utility of the strain co-displaying β-mannanase and β-mannosidase for ethanol fermentation from mannan biomass. Thus, co-tethering β-mannanase and β-mannosidase on the yeast cell surface provides a powerful platform technology for yeast fermentation toward the production of bioethanol and other biochemicals from lignocellulosic materials containing mannan components.
Related Links	https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-016-0600-4.pdf
Ending Page	15
Page Count	15
Starting Page	1
File Format	HTM / HTML
ISSN	27313654
DOI	10.1186/s13068-016-0600-4
Journal	Biotechnology for Biofuels and Bioproducts
Issue Number	1
Volume Number	9
Language	English
Publisher	BioMed Central
Publisher Date	2016-09-02
Access Restriction	Open
Subject Keyword	Biotechnology Plant Breeding Environmental Engineering Renewable and Green Energy Microbiology Saccharomyces cerevisiae Mannan Yeast Cell surface display Mannanase Mannosidase Ethanol Fermentation Biomass resource Biofuel Plant Breeding/Biotechnology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Energy Management, Monitoring, Policy and Law Renewable Energy, Sustainability and the Environment Applied Microbiology and Biotechnology Biotechnology
Journal Impact Factor	6.1/2023
5-Year Journal Impact Factor	6/2023

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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