NDLI: 4-Methylumbelliferone inhibits ovarian cancer growth by suppressing thymidine phosphorylase expression

Content Provider	Springer Nature : BioMed Central
Author	Tamura, Ryosuke Yokoyama, Yoshihito Yoshida, Hidemi Imaizumi, Tadaatsu Mizunuma, Hideki
Abstract	Background 4-Methylumbelliferone (4-MU), a hyaluronan (HA) synthesis inhibitor, has antitumor activity in cancer cells. However, few studies have focused on its effects on ovarian cancer. The aim of this study was to investigate the effects of 4-MU on ovarian cancer and to elucidate its mechanism of action. Methods The HRA human ovarian serous adenocarcinoma cell line was used in this study. The effects of 4-MU on cell proliferation, migration, and invasion were determined by using in vitro assays as well as an in vivo rat peritoneal carcinomatosis model. The expression of HA synthase (HAS), CD44 HA receptor, vascular endothelial growth factor (VEGF), and thymidine phosphorylase (TP) mRNA in HRA cells was analyzed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Results 4-MU administration inhibited the growth of peritoneal tumors and significantly prolonged survival. In vitro experiments showed that 4-MU inhibited HRA cell proliferation in a dose-dependent manner, while it did not affect HRA cell invasion and migration. 4-MU significantly decreased TP mRNA expression in HRA cells. On the other hand, since HAS2, CD44, and VEGF endogenous mRNA expression levels were very low in HRA cells, it was impossible to evaluate the effect of 4-MU treatment. Conclusions These results suggest that 4-MU exerts its antitumor effect on ovarian cancer through suppressing TP expression.
Related Links	https://ovarianresearch.biomedcentral.com/counter/pdf/10.1186/s13048-014-0094-2.pdf
Ending Page	8
Page Count	8
Starting Page	1
File Format	HTM / HTML
ISSN	17572215
DOI	10.1186/s13048-014-0094-2
Journal	Journal of Ovarian Research
Issue Number	1
Volume Number	7
Language	English
Publisher	BioMed Central
Publisher Date	2014-10-11
Access Restriction	Open
Subject Keyword	Gynecology Reproductive Medicine 4-Methylumbelliferone Ovarian cancer Peritonitis carcinomatosa Hymidine phosphorylase HRA cells
Content Type	Text
Resource Type	Article
Subject	Oncology Obstetrics and Gynecology
Journal Impact Factor	3.8/2023
5-Year Journal Impact Factor	4.2/2023

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4-Methylumbelliferone inhibits ovarian cancer growth by suppressing thymidine phosphorylase expression

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