NDLI: Evaluation of CX3CR1 gene DNA methylation in developmental dysplasia of the hip (DDH)

Content Provider	Springer Nature : BioMed Central
Author	Nejadhosseinian, Mohammad Haerian, Hoda Shirkoohi, Reza Karami, Jafar Mortazavi, Seyed Mohammad Javad
Abstract	Introduction and objective Developmental dysplasia of the hip (DDH) is a musculoskeletal disorder. Genetic and epigenetic changes in C-X3-C motif chemokine receptor 1 (CX3CR1) may lead to disturbance in chondrocyte development and change the labrum dimensions, which indirectly result in hip joint instability. Considering the important role of this gene in cell migration, cell adhesion and bone and cartilage development, we aimed to evaluate the CX3CR1 gene methylation in DDH pathogenesis. Methods Our study comprised of forty-five DDH patients and forty-five healthy control subjects with healthy femoral neck cartilage. The healthy controls had total or hemiarthroplasty for the femoral neck fracture. Samples were collected from the femoral head (cartilage) of DDH patients and healthy controls. Genomic DNA was obtained from the samples, and DNA methylation of CX3CR1 gene was analyzed via metabisulfite method. Results Methylation analysis reveals no significant differences in promoter of CX3CR1 gene in cartilage samples from DDH patients and healthy control subjects (P = 0.33). Conclusion Methylation status of CX3CR1 gene showed no significant difference between the patient and control groups. Our results indicate that DNA methylation may not modulate this gene in this disease and other epigenetic mechanisms such as non-coding RNAs and histone modifications could be implicated.
Related Links	https://josr-online.biomedcentral.com/counter/pdf/10.1186/s13018-022-03324-w.pdf
Ending Page	5
Page Count	5
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/s13018-022-03324-w
Journal	Journal of Orthopaedic Surgery and Research
Issue Number	1
Volume Number	17
Language	English
Publisher	BioMed Central
Publisher Date	2022-09-29
Access Restriction	Open
Subject Keyword	Orthopedics Surgical Orthopedics CX3CR1 gene DNA methylation Developmental dysplasia of the hip (DDH)
Content Type	Text
Resource Type	Article
Subject	Surgery Orthopedics and Sports Medicine
Journal Impact Factor	2.8/2023
5-Year Journal Impact Factor	3/2023

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