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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Mardini, Majd Kazancev, Mikhail Ivoilova, Elina Utkina, Victoria Vlasova, Anastasia Demurin, Yakov Soloviev, Alexander Kirov, Ilya |
| Abstract | Virus-Induced Gene Silencing (VIGS) is a versatile tool in plant science, yet its application to non-model species like sunflower demands extensive optimization due to transformation challenges. In this study, we aimed to elucidate the factors that significantly affect the efficiency of Agrobacterium-VIGS in sunflowers. After testing a number of approaches, we concluded that the seed vacuum technique followed by 6 h of co-cultivation produced the most efficient VIGS results. Genotype-dependency analysis revealed varying infection percentages (62–91%) and silencing symptom spreading in different sunflower genotypes. Additionally, we explored the mobility of tobacco rattle virus (TRV) and phenotypic silencing manifestation (photo-bleaching) across different tissues and regions of VIGS-infected sunflower plants. We showed the presence of TRV is not necessarily limited to tissues with observable silencing events. Finally, time-lapse observation demonstrated a more active spreading of the photo-bleached spots in young tissues compared to mature ones. This study not only offers a robust VIGS protocol for sunflowers but also provides valuable insights into genotype-dependent responses and the dynamic nature of silencing events, shedding light on TRV mobility across different plant tissues. |
| Related Links | https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-024-01241-z.pdf |
| Ending Page | 13 |
| Page Count | 13 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 17464811 |
| DOI | 10.1186/s13007-024-01241-z |
| Journal | Plant Methods |
| Issue Number | 1 |
| Volume Number | 20 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2024-08-12 |
| Access Restriction | Open |
| Subject Keyword | Plant Sciences Biological Techniques Helianthus annuus VIGS Sunflower PDS Agrobacterium Tobacco rattle virus Seeds Vacuum infiltration Simple protocol TRV mobility Genotype-dependency |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Biotechnology Genetics |
| Journal Impact Factor | 4.7/2023 |
| 5-Year Journal Impact Factor | 5.6/2023 |
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