NDLI: A novel method to characterize silica bodies in grasses

Content Provider	Springer Nature : BioMed Central
Author	Dabney, Clemon Ostergaard, Jason Watkins, Eric Chen, Changbin
Abstract	Background The deposition of silicon into epidermal cells of grass species is thought to be an important mechanism that plants use as a defense against pests and environmental stresses. There are a number of techniques available to study the size, density and distribution pattern of silica bodies in grass leaves. However, none of those techniques can provide a high-throughput analysis, especially for a great number of samples. Results We developed a method utilizing the autofluorescence of silica bodies to investigate their size and distribution, along with the number of carbon inclusions within the silica bodies of perennial grass species Koeleria macrantha. Fluorescence images were analyzed by image software Adobe Photoshop CS5 or ImageJ that remarkably facilitated the quantification of silica bodies in the dry ash. We observed three types of silica bodies or silica body related mineral structures. Silica bodies were detected on both abaxial and adaxial epidermis of K. macrantha leaves, although their sizes, density, and distribution patterns were different. No auto-fluorescence was detected from carbon inclusions. Conclusions The combination of fluorescence microscopy and image processing software displayed efficient utilization in the identification and quantification of silica bodies in K. macrantha leaf tissues, which should applicable to biological, ecological and geological studies of grasses including forage, turf grasses and cereal crops.
Related Links	https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-016-0108-8.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
ISSN	17464811
DOI	10.1186/s13007-016-0108-8
Journal	Plant Methods
Issue Number	1
Volume Number	12
Language	English
Publisher	BioMed Central
Publisher Date	2016-01-21
Access Restriction	Open
Subject Keyword	Plant Sciences Biological Techniques Koeleria macrantha Silica body Fluorescence microscopy Dry ash-imaging Photoshop Junegrass
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology Genetics
Journal Impact Factor	4.7/2023
5-Year Journal Impact Factor	5.6/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A novel method to characterize silica bodies in grasses

A customizable method to characterize Arabidopsis thaliana transpiration under drought conditions

A method for detecting single mRNA molecules in Arabidopsis thaliana

Koeleria macrantha (ledeb.) schultes.

A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in C3 and C4 grasses

Chlorophyll fluorescence imaging captures photochemical efficiency of grain sorghum (Sorghum bicolor) in a field setting

High spatio-temporal-resolution detection of chlorophyll fluorescence dynamics from a single chloroplast with confocal imaging fluorometer

A random-sampling approach to track cell divisions in time-lapse fluorescence microscopy

Hyperspectral imaging to characterize plant–plant communication in response to insect herbivory

A novel method to characterize silica bodies in grasses

Similar Documents

A novel method to characterize silica bodies in grasses

A customizable method to characterize Arabidopsis thaliana transpiration under drought conditions

A method for detecting single mRNA molecules in Arabidopsis thaliana

Koeleria macrantha (ledeb.) schultes.

A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in C3 and C4 grasses

Chlorophyll fluorescence imaging captures photochemical efficiency of grain sorghum (Sorghum bicolor) in a field setting

High spatio-temporal-resolution detection of chlorophyll fluorescence dynamics from a single chloroplast with confocal imaging fluorometer

A random-sampling approach to track cell divisions in time-lapse fluorescence microscopy

Hyperspectral imaging to characterize plant–plant communication in response to insect herbivory

A novel method to characterize silica bodies in grasses