NDLI: Analysis of breast milk fatty acid composition using dried milk samples

Content Provider	Springer Nature : BioMed Central
Author	Jackson, Kristina Harris Polreis, Jason Sanborn, Laura Chaima, David Harris, William S.
Abstract	Background The effect of breast milk fatty acid (FA) composition, particularly levels of docosahexaenoic acid (DHA), on infant health outcomes is unclear. Part of the reason for this is difficulties in collecting, storing and shipping milk samples to the laboratory. Here we report the validation of a dried milk spot (DMS) system to measure FA composition to help overcome these obstacles. Milk FA were measured by gas chromatography and reported as percent of total FA; the FA of primary interest in this study were DHA and industrially produced trans FA (iTFA). Experiments were carried out using pooled milk samples from US (n = 5) and Malawian women (n = 50). Experiments compared liquid vs. DMS samples (n = 55), assessed stability of FA composition under different storage conditions (n = 5), and compared the results from two different labs using the same methods (n = 5). Results Both % DHA and % iTFA levels in liquid and DMS samples were strongly correlated (R2 = 0.99 and 0.99, respectively, P < 0.0001). The % DHA in DMS samples was stable for up to four weeks at room temperature and up to three years at -80 °C; only slight deviations from the acceptable range of variability (±15 %) occurred in the 4 °C and -20 °C conditions for % DHA. The % iTFA was stable under all conditions. All % DHA and % iTFA were within 15 % of the referent when analyzed in two laboratories. Conclusions Valid FA composition values can be obtained from DMS samples using this robust collection and transport system which should facilitate studies of the role of milk FA composition in infant development.
Related Links	https://internationalbreastfeedingjournal.biomedcentral.com/counter/pdf/10.1186/s13006-016-0060-2.pdf
Ending Page	7
Page Count	7
Starting Page	1
File Format	HTM / HTML
ISSN	17464358
DOI	10.1186/s13006-016-0060-2
Journal	International Breastfeeding Journal
Issue Number	1
Volume Number	11
Language	English
Publisher	BioMed Central
Publisher Date	2016-01-25
Access Restriction	Open
Subject Keyword	Maternal and Child Health Breast milk Docosahexaenoic acid Assessment of nutritional status Lactation Infant and child nutrition Maternal nutrition
Content Type	Text
Resource Type	Article
Subject	Obstetrics and Gynecology Pediatrics, Perinatology and Child Health
Journal Impact Factor	2.9/2023
5-Year Journal Impact Factor	3.6/2023

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