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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Gao, Zhan Shao, Jun-Jun Zhang, Guang-Lei Ge, Su-Dan Chang, Yan-Yan Xiao, Lei Chang, Hui-Yun |
| Abstract | Background African swine fever (ASF), characterized by acute, severe, and fast-spreading, is a highly lethal swine infectious disease caused by the African swine fever virus (ASFV), which has caused substantial economic losses to the pig industry worldwide in the past 100 years. Methods This study started with bioinformatics methods and verified the epitope fusion protein method's reliability that does not rely on traditional epitope identification. Meanwhile, it will also express and purify the constructed genes through prokaryotic expression and establish antibody detection methods. Results The results indicated that the protein had good reactivity and did not cross-react with other swine diseases. The receiver-operating characteristic analysis was performed to verify the determination. The area under the receiver-operating characteristic curve was 0.9991 (95% confidence interval 0.9973 to 1.001). Conclusions It was proved that the recombinant protein is feasible as a diagnostic antigen to distinguish ASFV and provides a new idea for ASFV antibody detection. |
| Related Links | https://virologyj.biomedcentral.com/counter/pdf/10.1186/s12985-021-01568-2.pdf |
| Ending Page | 10 |
| Page Count | 10 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| DOI | 10.1186/s12985-021-01568-2 |
| Journal | Virology Journal |
| Issue Number | 1 |
| Volume Number | 18 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2021-05-05 |
| Access Restriction | Open |
| Subject Keyword | Virology African swine fever virus Indirect enzyme-linked immunosorbent assay Recombinant protein Multi-epitope |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Infectious Diseases |
| Journal Impact Factor | 4/2023 |
| 5-Year Journal Impact Factor | 3.8/2023 |
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