NDLI: Molecular characterization of Orf virus in goats in Gabon, Central Africa

Content Provider	Springer Nature : BioMed Central
Author	Maganga, Gael D. Relmy, Anthony Bakkali-Kassimi, Labib Ngoubangoye, Barthélémy Tsoumbou, Thierry Bouchier, Christiane N’Dilimabaka, Nadine Leroy, Eric M. Zientara, Stéphan Berthet, Nicolas
Abstract	Background Orf or contagious ecthyma is a zoonotic viral infection with a potential serious health threat for the small ruminants industry as well as humans. It is currently emerging in new territories. Results Eight suspected clinical cases of pustular dermatitis in goats occurred in the rural area of Tebe, in south-eastern Gabon, in January 2013. The orf virus (ORFV) was detected by high-throughput sequencing on sera, buccal swabs and scab pool samples. It was confirmed in six out of eight sick goats by using specific PCR targeting the major envelope protein (B2L) and the orf virus interferon resistance (VIR) genes. Phylogenetic analysis revealed that the Gabonese strain and South Korean strains evolved from a common ancestor, suggesting an Asian origin of the ORFV’ Gabonese strain. Conclusions This study provides the molecular detection of the ORFV strain involved in the cases of pustular dermatitis in goats and highlights its circulation in Gabon.
Related Links	https://virologyj.biomedcentral.com/counter/pdf/10.1186/s12985-016-0535-1.pdf
Ending Page	5
Page Count	5
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/s12985-016-0535-1
Journal	Virology Journal
Issue Number	1
Volume Number	13
Language	English
Publisher	BioMed Central
Publisher Date	2016-05-13
Access Restriction	Open
Subject Keyword	Virology Orf virus Goats High-throughput sequencing PCR
Content Type	Text
Resource Type	Article
Subject	Virology Infectious Diseases
Journal Impact Factor	4/2023
5-Year Journal Impact Factor	3.8/2023

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