NDLI: Group-specific cellular metabolism in Medulloblastoma

Content Provider	Springer Nature : BioMed Central
Author	Funke, Viktoria L. E. Walter, Carolin Melcher, Viktoria Wei, Lanying Sandmann, Sarah Hotfilder, Marc Varghese, Julian Jäger, Natalie Kool, Marcel Jones, David T. W. Pfister, Stefan M. Milde, Till Mynarek, Martin Rutkowski, Stefan Seggewiss, Jochen Jeising, Daniela de Faria, Flavia W. Marquardt, Thorsten Albert, Thomas K. Schüller, Ulrich Kerl, Kornelius
Abstract	Background Cancer metabolism influences multiple aspects of tumorigenesis and causes diversity across malignancies. Although comprehensive research has extended our knowledge of molecular subgroups in medulloblastoma (MB), discrete analysis of metabolic heterogeneity is currently lacking. This study seeks to improve our understanding of metabolic phenotypes in MB and their impact on patients’ outcomes. Methods Data from four independent MB cohorts encompassing 1,288 patients were analysed. We explored metabolic characteristics of 902 patients (ICGC and MAGIC cohorts) on bulk RNA level. Moreover, data from 491 patients (ICGC cohort) were searched for DNA alterations in genes regulating cell metabolism. To determine the role of intratumoral metabolic differences, we examined single-cell RNA-sequencing (scRNA-seq) data from 34 additional patients. Findings on metabolic heterogeneity were correlated to clinical data. Results Established MB groups exhibit substantial differences in metabolic gene expression. By employing unsupervised analyses, we identified three clusters of group 3 and 4 samples with distinct metabolic features in ICGC and MAGIC cohorts. Analysis of scRNA-seq data confirmed our results of intertumoral heterogeneity underlying the according differences in metabolic gene expression. On DNA level, we discovered clear associations between altered regulatory genes involved in MB development and lipid metabolism. Additionally, we determined the prognostic value of metabolic gene expression in MB and showed that expression of genes involved in metabolism of inositol phosphates and nucleotides correlates with patient survival. Conclusion Our research underlines the biological and clinical relevance of metabolic alterations in MB. Thus, distinct metabolic signatures presented here might be the first step towards future metabolism-targeted therapeutic options. Graphical Abstract
Related Links	https://translational-medicine.biomedcentral.com/counter/pdf/10.1186/s12967-023-04211-6.pdf
Ending Page	16
Page Count	16
Starting Page	1
File Format	HTM / HTML
ISSN	14795876
DOI	10.1186/s12967-023-04211-6
Journal	Journal of Translational Medicine
Issue Number	1
Volume Number	21
Language	English
Publisher	BioMed Central
Publisher Date	2023-06-05
Access Restriction	Open
Subject Keyword	Biomedicine Medicine Public Health Medulloblastoma Metabolism Inositol phosphates Nucleotides RNA-Seq Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology Medicine
Journal Impact Factor	6.1/2023
5-Year Journal Impact Factor	6.3/2023

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