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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Zhu, Qi Yao, Simin Dong, Yishan Liu, Dan Wang, Huiyan Jiang, Peipei Dai, Chenyan Lv, Haining Cao, Chenrui Zhou, Zhenhua Wang, Limin Gou, Wenjing Zhang, Xiwen Zhao, Guangfeng Hu, Yali |
| Abstract | Background Thin endometrium (TE) is a challenging clinical issue in the reproductive medicine characterized by inadequate endometrial thickness, poor response to estrogen and no effective treatments currently. At present, the precise pathogenesis of thin endometria remains to be elucidated. We aimed to explore the related molecular mechanism of TE by comparing the transcriptome profiles of late-proliferative phase endometria between TE and matched controls. Methods We performed a bulk RNA-Seq (RNA-sequencing) of endometrial tissues in the late-proliferative phase in 7 TE and 7 matched controls for the first time. Differential gene expression analysis, gene ontology enrichment analysis and protein-protein interactions (PPIs) network analysis were performed. Immunohistochemistry was used for molecular expression and localization in endometria. Human endometrial stromal cells (HESCs) were isolated and cultured for verifying the functions of hub gene. Results Integrative data mining of our RNA-seq data in endometria revealed that most genes related to cell division and cell cycle were significantly inhibited, while inflammation activation, immune response and reactive oxygen species associated genes were upregulated in TE. PBK was identified as a hub of PPIs network, and its expression level was decreased by 2.43-fold in endometria of TE patients, particularly reduced in the stromal cells, which was paralleled by the decreased expression of Ki67. In vitro experiments showed that the depletion of PBK reduced the proliferation of HESCs by 50% and increased the apoptosis of HESCs by 1 time, meanwhile PBK expression was inhibited by oxidative stress (reduced by 76.2%), hypoxia (reduced by 51.9%) and inflammatory factors (reduced by approximately 50%). These results suggested that the insufficient expression of PBK was involved in the poor endometrial thickness in TE. Conclusions The endometrial transcriptome in late-proliferative phase showed suppressed cell proliferation in women with thin endometria and decreased expression of PBK in human endometrial stromal cells (HESCs), to which inflammation and reactive oxygen species contributed. |
| Related Links | https://rbej.biomedcentral.com/counter/pdf/10.1186/s12958-022-00903-8.pdf |
| Ending Page | 13 |
| Page Count | 13 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14777827 |
| DOI | 10.1186/s12958-022-00903-8 |
| Journal | Reproductive Biology and Endocrinology |
| Issue Number | 1 |
| Volume Number | 20 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2022-02-02 |
| Access Restriction | Open |
| Subject Keyword | Reproductive Medicine Endocrinology Thin endometrium RNA-seq Transcriptome PBK TOPK HESCs |
| Content Type | Text |
| Resource Type | Article |
| Subject | Endocrinology Obstetrics and Gynecology Developmental Biology Reproductive Medicine |
| Journal Impact Factor | 4.2/2023 |
| 5-Year Journal Impact Factor | 5.3/2023 |
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