NDLI: Label-free proteomics uncovers SMC1A expression is Down-regulated in AUB-E

Content Provider	Springer Nature : BioMed Central
Author	Jia, Yingxian Luo, Jie Lan, Yibing Li, Chunming Ma, Linjuan Zhu, Xiaoming Ruan, Fei Zhou, Jianhong
Abstract	Background While heavy menstrual bleeding (HMB) is a prevalent symptom among women with abnormal uterine bleeding caused by endometrial disorder (AUB-E) seeking gynecologic care, the primary endometrial disorder remains poorly understood. Methods Five human endometrial samples from women with AUB-E and the age-matched healthy women were selected, respectively. Proteins from the samples were analyzed by a linear ion trap (LTQ)-Orbitrap Elite mass spectrometer based label-free proteomic approach. The purpose protein was validated by western blot and immunohistochemistry staining. Results A total of 2353 protein groups were quantified under highly stringent criteria with a false discovery rate of < 1% for protein groups, and 291 differentially expressed proteins were significantly changed between the two groups. The results showed that the down-regulation of structural maintenance of chromosomes protein 1A (SMC1A) in AUB-E patients. Next, this change in the glandular epithelial cells was validated by immunohistochemistry. Conclusion The results indicated a novel mechanism for the cause of AUB-E, as down-expression SMC1A potentially regulated the cell cycle progression in endometrial glandular epithelium further led to bleeding.
Related Links	https://rbej.biomedcentral.com/counter/pdf/10.1186/s12958-021-00713-4.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
ISSN	14777827
DOI	10.1186/s12958-021-00713-4
Journal	Reproductive Biology and Endocrinology
Issue Number	1
Volume Number	19
Language	English
Publisher	BioMed Central
Publisher Date	2021-03-02
Access Restriction	Open
Subject Keyword	Reproductive Medicine Endocrinology Abnormal uterine bleeding Human endometrium Proteomic analysis Primary endometrial disorder
Content Type	Text
Resource Type	Article
Subject	Endocrinology Obstetrics and Gynecology Developmental Biology Reproductive Medicine
Journal Impact Factor	4.2/2023
5-Year Journal Impact Factor	5.3/2023

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