NDLI: Neuron-derived exosomes-transmitted miR-124-3p protect traumatically injured spinal cord by suppressing the activation of neurotoxic microglia and astrocytes

Content Provider	Springer Nature : BioMed Central
Author	Jiang, Dongdong Gong, Fangyi Ge, Xuhui Lv, Chengtang Huang, Chenyu Feng, Shuang Zhou, Zheng Rong, Yuluo Wang, Jiaxing Ji, Chengyue Chen, Jian Zhao, Wene Fan, Jin Liu, Wei Cai, Weihua
Abstract	Background Spinal cord injury (SCI) is a catastrophic injury that can cause irreversible motor dysfunction with high disability. Exosomes participate in the transport of miRNAs and play an essential role in intercellular communication via transfer of genetic material. However, the miRNAs in exosomes which derived from neurons, and the underlying mechanisms by which they contribute to SCI remain unknown. Methods A contusive in vivo SCI model and a series of in vitro experiments were carried out to explore the therapeutic effects of exosomes. Then, a miRNA microarray analysis and rescue experiments were performed to confirm the role of neuron-derived exosomal miRNA in SCI. Western blot, luciferase activity assay, and RNA-ChIP were used to investigate the underlying mechanisms. Results The results indicated that neuron-derived exosomes promoted functional behavioral recovery by suppressing the activation of M1 microglia and A1 astrocytes in vivo and in vitro. A miRNA array showed miR-124-3p to be the most enriched in neuron-derived exosomes. MYH9 was identified as the target downstream gene of miR-124-3p. A series of experiments were used to confirm the miR-124-3p/MYH9 axis. Finally, it was found that PI3K/AKT/NF-κB signaling cascades may be involved in the modulation of microglia by exosomal miR-124-3p. Conclusion A combination of miRNAs and neuron-derived exosomes may be a promising, minimally invasive approach for the treatment of SCI.
Related Links	https://jnanobiotechnology.biomedcentral.com/counter/pdf/10.1186/s12951-020-00665-8.pdf
Ending Page	20
Page Count	20
Starting Page	1
File Format	HTM / HTML
ISSN	14773155
DOI	10.1186/s12951-020-00665-8
Journal	Journal of Nanobiotechnology
Issue Number	1
Volume Number	18
Language	English
Publisher	BioMed Central
Publisher Date	2020-07-25
Access Restriction	Open
Subject Keyword	Biotechnology Nanotechnology Molecular Medicine Spinal cord injury Exosomes Microglia Astrocytes miR-124-3p/MYH9 axis
Content Type	Text
Resource Type	Article
Subject	Bioengineering Pharmaceutical Science Medicine Applied Microbiology and Biotechnology Biomedical Engineering Molecular Medicine Nanoscience and Nanotechnology
Journal Impact Factor	10.6/2023
5-Year Journal Impact Factor	11.4/2023

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