NDLI: The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis

Content Provider	Springer Nature : BioMed Central
Author	Chen, Qing Zhang, Xiaowei Hu, Qicai Zhang, Wei Xie, Yi Wei, Weixia
Abstract	Background Chronic endometritis (CE) is a disease of continuous and subtle inflammation occurring in the endometrial stromal area, which is often asymptomatic or present with non-specific clinical symptoms. Methods This study investigated the composition and distribution of the intrauterine microbiota of 71 patients who underwent hysteroscopy during the routine clinical inspection of infertility. Among them, patients who were diagnosed with chronic endometritis (CE) were allocated into CE group (n = 29) and others into non-CE group (n = 42). There was no significant difference in average age between the two groups (P = 0.19). Uterine flushing fluid was collected by the self-developed cervical trocar uterine cavity sampler and 16S rRNA sequencing was performed. Results The alpha diversity in the CE group was significantly higher than that in the non-CE group (P < 0.05). Firmicutes (newly named Bacillota) were the dominant phylum in the non-CE group (72.23%), while their abundance was much lower in the CE group (49.92%), but there was no statistically significant difference between the two groups. The abundances of Actinobacteriota and Cyanobacteria in the CE group were significantly higher than those in the non-CE group (P < 0.05). At the genus level, the abundance of Lactobacillus dominated in all samples, which presented a significantly lower abundance in the CE group (40.88%) than that in the non-CE group (64.22%) (P < 0.05). Correspondingly, the abundance of non-Lactobacillus was higher in the CE group, among which Pseudomonas and Cutibacterium increased significantly (P < 0.01). Moreover, compared with the non-CE group, the pathways involved in arginine and proline metabolism and retinol metabolism were significantly enriched in the CE group (P < 0.05), while the metabolism of lipid and prenyltransferases were significantly decreased in the CE group (P < 0.05). Conclusions A certain microbial community was colonized in the uterine cavity, which was dominated by Lactobacillus. The structure and distribution of intrauterine microbiota in the CE group were different from those in the non-CE group by showing a lower abundance of Lactobacillus, and a significantly higher abundance of Pseudomonas and Cutibacterium. Additionally, the microbial metabolism was altered in the CE group. This study elaborated the alteration of intrauterine microbiota in CE patients, which may contribute to the diagnosis of CE and provide a reference for antibiotic treatment of CE.
Related Links	https://ann-clinmicrob.biomedcentral.com/counter/pdf/10.1186/s12941-023-00556-4.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
ISSN	14760711
DOI	10.1186/s12941-023-00556-4
Journal	Annals of Clinical Microbiology and Antimicrobials
Issue Number	1
Volume Number	22
Language	English
Publisher	BioMed Central
Publisher Date	2023-01-12
Access Restriction	Open
Subject Keyword	Medical Microbiology Infectious Diseases Drug Resistance Microbial Genetics and Genomics Biochemistry Parasitology Lactobacillus Chronic endometritis Intrauterine microbiota 16S rRNA sequencing Firmicutes (Bacillota)
Content Type	Text
Resource Type	Article
Subject	Microbiology (medical) Infectious Diseases
Journal Impact Factor	4.6/2023
5-Year Journal Impact Factor	5.3/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Dysbiosis of urine microbiota in obstructive urinary retention patients revealed by next-generation sequencing

Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure

Microbiota composition and distribution along the female reproductive tract of women with endometriosis

Effect of β-lactam antibiotics on the gut microbiota of term neonates

Extraction of total nucleic acids from bacterial isolates using the bioMérieux NucliSENS easyMAG total nucleic acid extractor

Diagnosis of pathogens causing bacterial meningitis using Nanopore sequencing in a resource-limited setting

Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses

Exploring internal features of 16S rRNA gene for identification of clinically relevant species of the genus Streptococcus

The impact of urine microbiota in patients with lower urinary tract symptoms

The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis

Similar Documents

Dysbiosis of urine microbiota in obstructive urinary retention patients revealed by next-generation sequencing

Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure

Microbiota composition and distribution along the female reproductive tract of women with endometriosis

Effect of β-lactam antibiotics on the gut microbiota of term neonates

Extraction of total nucleic acids from bacterial isolates using the bioMérieux NucliSENS easyMAG total nucleic acid extractor

Diagnosis of pathogens causing bacterial meningitis using Nanopore sequencing in a resource-limited setting

Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses

Exploring internal features of 16S rRNA gene for identification of clinically relevant species of the genus Streptococcus

The impact of urine microbiota in patients with lower urinary tract symptoms

The alteration of intrauterine microbiota in chronic endometritis patients based on 16S rRNA sequencing analysis