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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Gonçalves, Ana Alice Maia Ribeiro, Anna Julia Resende, Carlos Ananias Aparecido Couto, Carolina Alves Petit Gandra, Isadora Braga dos Santos Barcelos, Isabelle Caroline da Silva, Jonatas Oliveira Machado, Juliana Martins Silva, Kamila Alves Silva, Líria Souza dos Santos, Michelli da Silva Lopes, Lucas de Faria, Mariana Teixeira Pereira, Sabrina Paula Xavier, Sandra Rodrigues Aragão, Matheus Motta Candida-Puma, Mayron Antonio de Oliveira, Izadora Cristina Moreira Souza, Amanda Araujo Nogueira, Lais Moreira da Paz, Mariana Campos Coelho, Eduardo Antônio Ferraz Giunchetti, Rodolfo Cordeiro de Freitas, Sonia Maria Chávez-Fumagalli, Miguel Angel Nagem, Ronaldo Alves Pinto Galdino, Alexsandro Sobreira |
| Abstract | Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide application in the most diverse diseases, this review article aims to survey the use of these antigens for diagnosis, as well as discuss the main points surrounding these antigens. RMPs usually consisting of linear, immunodominant, and phylogenetically conserved epitopes, has been applied in the experimental diagnosis of various human and animal diseases, such as leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis, leptospirosis, leprosy, filariasis, schistosomiasis, dengue, and COVID-19. The synthetic genes for these epitopes are joined to code a single RMP, either with spacers or fused, with different biochemical properties. The epitopes’ high density within the RMPs contributes to a high degree of sensitivity and specificity. The RMPs can also sidestep the need for multiple peptide synthesis or multiple recombinant proteins, reducing costs and enhancing the standardization conditions for immunoassays. Methods such as bioinformatics and circular dichroism have been widely applied in the development of new RMPs, helping to guide their construction and better understand their structure. Several RMPs have been expressed, mainly using the Escherichia coli expression system, highlighting the importance of these cells in the biotechnological field. In fact, technological advances in this area, offering a wide range of different strains to be used, make these cells the most widely used expression platform. RMPs have been experimentally used to diagnose a broad range of illnesses in the laboratory, suggesting they could also be useful for accurate diagnoses commercially. On this point, the RMP method offers a tempting substitute for the production of promising antigens used to assemble commercial diagnostic kits. |
| Related Links | https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-024-02418-w.pdf |
| Ending Page | 32 |
| Page Count | 32 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14752859 |
| DOI | 10.1186/s12934-024-02418-w |
| Journal | Microbial Cell Factories |
| Issue Number | 1 |
| Volume Number | 23 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2024-05-22 |
| Access Restriction | Open |
| Subject Keyword | Applied Microbiology Biotechnology Microbiology Microbial Genetics and Genomics Enzymology Genetic Engineering Escherichia coli Recombinant multiepitope proteins Bioinformatics Biophysical analysis Immunodiagnosis |
| Content Type | Text |
| Resource Type | Review |
| Subject | Applied Microbiology and Biotechnology Bioengineering Biotechnology |
| Journal Impact Factor | 4.3/2023 |
| 5-Year Journal Impact Factor | 5.5/2023 |
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