NDLI: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

Content Provider	Springer Nature : BioMed Central
Author	Kim, Jung-Hun Wang, Chonglong Jang, Hui-Jung Cha, Myeong-Seok Park, Ju-Eon Jo, Seon-Yeong Choi, Eui-Sung Kim, Seon-Won
Abstract	Background Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives. Results In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80 mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337 mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (Ptrc), which resulted in an additional increase in isoprene production up to 956 mg/L. To reduce the formation of byproducts derived from acetyl-CoA (an initial substrate of the MVA pathway), nine relevant genes were deleted to generate the E. coli AceCo strain (E. coli MG1655 ΔackA-pta, poxB, ldhA, dld, adhE, pps, and atoDA). The AceCo strain harboring the ispS gene and MVA pathway showed enhanced isoprene production of 1832 mg/L in flask culture with reduced accumulation of byproducts. Conclusions We achieved a 23-fold increase in isoprene production by codon optimization of PtispS, augmentation of the MVA pathway, and deletion of genes involved in byproduct formation.
Related Links	https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-016-0612-6.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
ISSN	14752859
DOI	10.1186/s12934-016-0612-6
Journal	Microbial Cell Factories
Issue Number	1
Volume Number	15
Language	English
Publisher	BioMed Central
Publisher Date	2016-12-23
Access Restriction	Open
Subject Keyword	Applied Microbiology Biotechnology Microbiology Microbial Genetics and Genomics Enzymology Genetic Engineering Escherichia coli Bioisoprene Mevalonate pathway Isoprene synthase Carbon utilization
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology Bioengineering Biotechnology
Journal Impact Factor	4.3/2023
5-Year Journal Impact Factor	5.5/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production

Fermentative production and direct extraction of (−)-α-bisabolol in metabolically engineered Escherichia coli

Study on the isoprene-producing co-culture system of Synechococcus elongates–Escherichia coli through omics analysis

Combinatory optimization of chromosomal integrated mevalonate pathway for β-carotene production in Escherichia coli

Engineering a non-oxidative glycolysis pathway in escherichia coli for high-level citramalate production

Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis

Enzymatic process optimization for the in vitro production of isoprene from mevalonate

Engineering Escherichia coli via introduction of the isopentenol utilization pathway to effectively produce geranyllinalool

GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

Similar Documents

Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production

Fermentative production and direct extraction of (−)-α-bisabolol in metabolically engineered Escherichia coli

Study on the isoprene-producing co-culture system of Synechococcus elongates–Escherichia coli through omics analysis

Combinatory optimization of chromosomal integrated mevalonate pathway for β-carotene production in Escherichia coli

Engineering a non-oxidative glycolysis pathway in escherichia coli for high-level citramalate production

Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis

Enzymatic process optimization for the in vitro production of isoprene from mevalonate

Engineering Escherichia coli via introduction of the isopentenol utilization pathway to effectively produce geranyllinalool

GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts