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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Sales, Leilane Pezuk, Julia Alejandra Borges, Kleiton Silva Brassesco, María Sol Scrideli, Carlos Alberto Tone, Luiz Gonzaga Santos, Marcelo Henrique dos Ionta, Marisa Oliveira, Jaqueline Carvalho de |
| Abstract | Background Glioblastoma is the most common tumor of the central nervous system and one of the hardest tumors to treat. Consequently, the search for novel therapeutic options is imperative. 7-epiclusianone, a tetraprenylated benzophenone isolated from the epicarp of the native plant Garcinia brasiliensis, exhibits a range of biological activities but its prospect anticancer activity is underexplored. Thus, the aim of the present study was to evaluate the influence of 7-epiclusianone on proliferation, clonogenic capacity, cell cycle progression and induction of apoptosis in two glioblastoma cell lines (U251MG and U138MG). Methods Cell viability was measured by the MTS assay; for the clonogenic assay, colonies were stained with Giemsa and counted by direct visual inspection; For cell cycle analysis, cells were stained with propidium iodide and analyzed by cytometry; Cyclin A expression was determined by immunoblotting; Apoptotic cell death was determined by annexin V fluorescein isothiocyanate labeling and Caspase-3 activity in living cells. Results Viability of both cell lines was drastically inhibited; moreover, the colony formation capacity was significantly reduced, demonstrating long-term effects even after removal of the drug. 7-epiclusianone treatment at low concentrations also altered cell cycle progression, decreased the S and G2/M populations and at higher concentrations increased the number of cells at sub-G1, in concordance with the increase of apoptotic cells. Conclusion The present study demonstrates for the first time the anticancer potential of 7-epiclusianone against glioblastoma cells, thus meriting its further investigation as a potential therapeutic agent. |
| Related Links | https://bmccomplementmedtherapies.biomedcentral.com/counter/pdf/10.1186/s12906-015-0911-1.pdf |
| Ending Page | 8 |
| Page Count | 8 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 26627671 |
| DOI | 10.1186/s12906-015-0911-1 |
| Journal | BMC Complementary Medicine and Therapies |
| Issue Number | 1 |
| Volume Number | 15 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2015-10-30 |
| Access Restriction | Open |
| Subject Keyword | Complementary & Alternative Medicine Internal Medicine Chiropractic Medicine 7-epiclusianone Glioblastoma Viability Apoptosis Cell cycle |
| Content Type | Text |
| Resource Type | Article |
| Subject | Complementary and Alternative Medicine |
| Journal Impact Factor | 3.3/2023 |
| 5-Year Journal Impact Factor | 3.6/2023 |
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