NDLI: Does the uterine microbiota affect the reproductive outcomes in women with recurrent implantation failures?

Content Provider	Springer Nature : BioMed Central
Author	Keburiya, Lela K. Smolnikova, Veronika Yu. Priputnevich, Tatiana V. Muravieva, Vera V. Gordeev, Alexey B. Trofimov, Dmitry Yu. Shubina, Ekaterina S. Kochetkova, Taisiya O. Rogacheva, Margarita S. Kalinina, Elena A. Sukhikh, Gennady T.
Abstract	Background Inefficiency of in vitro fertilization (IVF) programs can be caused by implantation failures. The uterine microbiota can influence the implantation process. However, it still remains unclear whether opportunistic microorganisms detected in the endometrium have a negative impact on the implantation success. The aim of our study was to evaluate the influence of the uterine microbiota on the embryo implantation success in patients undergoing assisted reproductive technologies. Methods The study included 130 women diagnosed with infertility. The patients were divided into three groups: group I included women with the first IVF attempt (n = 39); group II included patients with recurrent implantation failure following embryo transfer with ovarian stimulation (n = 27); group III consisted of women with recurrent implantation failure following frozen-thawed embryo transfer (n = 64). We performed microbiological examination of the embryo transfer catheter which was removed from the uterine cavity after embryo transfer; cervical discharge of all the patients was studied as well. Thirty patients were selected for metagenomic sequencing. Results The study showed that the uterine cavity is not free of microorganisms. A total of 44 species of microorganisms were detected: 26 species of opportunistic organisms and 18 species of commensals (14 species of lactobacilli and 4 species of bifidobacteria). Obligate anaerobic microorganisms and Gardnerella vaginalis were detected more frequently in group I compared to group III (strict anaerobes—15.4 and 1.6%; G. vaginalis—12.8 and 1.6%, respectively) (p < 0.05). However, this fact did not have a negative influence on the pregnancy rate: it was 51.3% in group I, it was 29.6% and 35.9% in women with recurrent implantation failures, respectively. Conclusion Opportunistic microorganisms which were revealed in low or moderate titers (103–105 CFU/ml) in the uterine cavity and cervical canal did not affect the pregnancy rate in the women in the study groups. The microflora of the uterine cavity and cervical canal differed in qualitative composition in 87.9% of patients, therefore, we can suggest that the uterine cavity may form its own microbiota. The microbiota of the uterine cavity is characterized by fewer species diversity compared to the microbiota of the cervical canal.
Related Links	https://bmcwomenshealth.biomedcentral.com/counter/pdf/10.1186/s12905-022-01750-w.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
ISSN	14726874
DOI	10.1186/s12905-022-01750-w
Journal	BMC Women's Health
Issue Number	1
Volume Number	22
Language	English
Publisher	BioMed Central
Publisher Date	2022-05-14
Access Restriction	Open
Subject Keyword	Gynecology Maternal and Child Health Reproductive Medicine Infertility Assisted reproductive technologies Uterine microbiota Metagenomic sequencing Embryo implantation
Content Type	Text
Resource Type	Article
Subject	Obstetrics and Gynecology Reproductive Medicine
Journal Impact Factor	2.4/2023
5-Year Journal Impact Factor	2.9/2023

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Correction to: Does the uterine microbiota affect the reproductive outcomes in women with recurrent implantation failures?

Distinct gut and vaginal microbiota profile in women with recurrent implantation failure and unexplained infertility

Uterine fluid microRNAs are dysregulated in women with recurrent implantation failure

Factors influencing endometrial receptivity in women with recurrent implantation failure

Sequential embryo transfer combined with intrauterine perfusion improved pregnancy outcomes in patients with recurrent implantation failure

Rationale and design of two prospective, multicenter, observational studies on reproductive outcome in women with recurrent failures after spontaneous or assisted conception: OTTILIA and FIRST registries

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Does the uterine microbiota affect the reproductive outcomes in women with recurrent implantation failures?