| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Kocaoğlu, Merve Hayriye Çubukçuoğlu Deniz, Günseli Aras-Tosun, Duru Altuntaş, Evrim Güneş Tuncay, Erkan |
| Abstract | Background Osteomyelitis involves bone destruction, impaired bone formation, and systemic inflammation. Dexmedetomidine (DXMT) possesses antioxidant, anti-inflammatory, and anti-apoptotic properties alongside sedative and analgesic effects. This study evaluates DXMT's effects on markers of infection and bone healing using osteocyte-like cells infected by Staphylococcus aureus (S. aureus). Methods Human osteosarcoma-derived SAOS-2 cells were differentiated to an osteocyte-like phenotype over 28 days using potassium dihydrogen phosphate. Differentiation was verified via qPCR for osteogenic markers. Cytotoxicity of DXMT (0.1–10 µM) was tested using WST-1 assay and Reactive Oxygen Species (ROS) production analysis. Cells infected with S. aureus were treated with DXMT to assess its antimicrobial, anti-inflammatory (via ELISA for cytokines IL1-ß, TNF-⍺, IL-17, and IL-6), and osteogenesis-promoting effects. Results DXMT ≤ 1 µM did not affect cell viability, while 2, 5, and 10 µM DXMT administration reduced cell counts. A 5 µM dose slightly reduced intracellular bacterial load (6.2 log in controls vs. 6.1 log with DXMT), while neither less nor more DXMT was effective on reducing the S. aureus load. Doses ≥ 5 µM effectively reduced ROS production and inflammation post-infection in a time-dependent manner. S. aureus infection decreased osteogenic markers, but DXMT mitigated cellular stress and inflammation with a positive impact on osteogenesis at therapeutic doses. Conclusion DXMT at 5 µM is an optimal dose to reduce infection-induced cellular stress and promote bone healing in osteomyelitis in vitro, balancing antimicrobial effects and cytotoxicity. |
| Related Links | https://bmcmusculoskeletdisord.biomedcentral.com/counter/pdf/10.1186/s12891-025-08555-6.pdf |
| Ending Page | 10 |
| Page Count | 10 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14712474 |
| DOI | 10.1186/s12891-025-08555-6 |
| Journal | BMC Musculoskeletal Disorders |
| Issue Number | 1 |
| Volume Number | 26 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2025-04-02 |
| Access Restriction | Open |
| Subject Keyword | Orthopedics Rehabilitation Rheumatology Sports Medicine Internal Medicine Epidemiology Staphylococcus aureus (S. aureus) Anti-inflammation Antimicrobial Dexmedetomidine Osteoblast Osteomyelitis Reactive Oxygen Species (ROS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Orthopedics and Sports Medicine Rheumatology |
| Journal Impact Factor | 2.2/2023 |
| 5-Year Journal Impact Factor | 2.6/2023 |
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