NDLI: Cells of renin lineage express hypoxia inducible factor 2α following experimental ureteral obstruction

Content Provider	Springer Nature : BioMed Central
Author	Stefanska, Ania Eng, Diana Kaverina, Natalya Pippin, Jeffrey W. Gross, Kenneth W. Duffield, Jeremy S. Shankland, Stuart J.
Abstract	Background Recent studies indicate that mural cells of the preglomerular vessels, known as cells of renin lineage (CoRL), contribute to repair and regeneration of injured kidney glomeruli. However, their potential roles in tubulointerstitial disease are less understood. The aim of this study was to better understand CoRL number and distribution following UUO so that future mechanistic studies could be undertaken. Methods We mapped the fate of CoRL in adult Ren1cCreER x Rs-tdTomato-R reporter mice that underwent UUO. Kidney biopsies from sham and UUO-subjected mice on days 3, 7, and 14 were evaluated by immunohistochemistry. Results In sham animals, CoRL were restricted to juxtaglomerular location. At day 7 following UUO, CoRL increased two-fold, were perivascular in location, and co-expressed pericyte markers (PDGFßR, NG2), but did not express renin. At day 14 post UUO, labeled CoRL detached from vessels and were present in the interstitium, in areas of fibrosis, where they now expressed the myofibroblast marker alpha-smooth muscle actin. The increase in CoRL was likely due to proliferation as marked by BrdU labeling, and migration from the cortex. Following UUO starting from day 3, active hypoxia inducible factor-2α was detected in nuclei in labeled CoRL, in the cortex, but not those cells found in medulla. Conclusions We have demonstrated that arteriolar CoRL are potential kidney progenitors that may contribute to the initial vascular regeneration. However, in chronic kidney injury (≥14 days post UUO), perivascular CoRL transition to myofibroblast-like cells.
Related Links	https://bmcnephrol.biomedcentral.com/counter/pdf/10.1186/s12882-015-0216-0.pdf
Ending Page	11
Page Count	11
Starting Page	1
File Format	HTM / HTML
ISSN	14712369
DOI	10.1186/s12882-015-0216-0
Journal	BMC Nephrology
Issue Number	1
Volume Number	17
Language	English
Publisher	BioMed Central
Publisher Date	2016-01-08
Access Restriction	Open
Subject Keyword	Nephrology Internal Medicine HIF-2α UUO Microvascular rarefaction Pericytes progenitor Tubulointerstitial PDGFßR Fibrosis Hypoxia
Content Type	Text
Resource Type	Article
Subject	Nephrology
Journal Impact Factor	2.2/2023
5-Year Journal Impact Factor	2.6/2023

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