NDLI: Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Content Provider	Springer Nature : BioMed Central
Author	Vidyagina, Elena O. Subbotina, Natalia M. Belyi, Vladimir A. Lebedev, Vadim G. Krutovsky, Konstantin V. Shestibratov, Konstantin A.
Abstract	Background Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them. Results Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
Related Links	https://bmcplantbiol.biomedcentral.com/counter/pdf/10.1186/s12870-020-02469-2.pdf
Ending Page	14
Page Count	14
Starting Page	1
File Format	HTM / HTML
ISSN	14712229
DOI	10.1186/s12870-020-02469-2
Journal	BMC Plant Biology
Issue Number	1
Volume Number	20
Language	English
Publisher	BioMed Central
Publisher Date	2020-06-03
Access Restriction	Open
Subject Keyword	Plant Sciences Agriculture Tree Biology Penicillium canescens Populus tremula Aspen Gene expression level Xyloglucanase Transgenic
Content Type	Text
Resource Type	Article
Subject	Plant Science
Journal Impact Factor	4.3/2023
5-Year Journal Impact Factor	5.2/2023

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Phenotypic manifestation of gene expression encoding xyloglucanase from Penicillium canescens in transgenic aspen plants

Overexpression of the recombinant xyloglucanase sp-Xeg from Penicillium canescens accelerates growth and rooting of transgenic aspen plants

Expression of xyloglucanase sp-Xeg gene from Penicillium canescens accelerates growth and rooting in transgenic aspen plants

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Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

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