NDLI: Genetic effect of an InDel in the promoter region of the NUDT15 and its effect on myoblast proliferation in chickens

Content Provider	Springer Nature : BioMed Central
Author	Wei, Chengjie Niu, Yufang Chen, Bingjie Qin, Panpan Wang, Yanxing Hou, Dan Li, Tong Li, Ruiting Wang, Chunxiu Yin, Huadong Han, Ruili Xu, Huifen Tian, Yadong Liu, Xiaojun Kang, Xiangtao Li, Zhuanjian
Abstract	Background Molecular breeding accelerates the speed of animal breeding. Screening molecular markers that can affect economic traits through genome-wide association studies (GWAS) can provide a theoretical basis for molecular breeding. At present, a large number of molecular markers have been screened in poultry research, but few reports on how molecular markers affect economic traits exist. It is particularly important to reveal the action mechanisms of molecular markers, which can provide more accurate information for molecular breeding. Results The aim of this study was to investigate the relationships between two indels (NUDT15-indel-2777 and NUDT15-indel-1673) in the promoter region of NUDT15 and growth and carcass traits in chickens and to explore the regulatory mechanism of NUDT15. Significant differences were found in genotype and allele frequencies among commercial broilers, commercial laying hens and dual-purpose chickens. The results of association analyses showed that these two indel loci could significantly affect growth traits, such as body weight, and carcass traits. Tissue expression profiling at E12 showed that the expression of NUDT15 was significantly higher in skeletal muscle, and time-expression profiling of leg muscle showed that the expression of NUDT15 in myoblasts was significantly higher in the E10 and E12 proliferation stages than in other stages. Promoter activity analysis showed that pro-1673-I and pro-1673-D significantly inhibited promoter activity, and the promoter activity of pro-1673-D was significantly lower than that of pro-1673-I. In addition, when NUDT15 was overexpressed or underwent interference in chicken primary myoblasts (CPMs), NUDT15 could inhibit the proliferation of CPMs. Conclusion The results suggest that the studied indels in the promoter region of NUDT15 may regulate the proliferation of CPMs by affecting NUDT15 expression, ultimately affecting the growth and carcass traits of chickens. These indel polymorphisms may be used together as molecular markers for improving economic traits in chickens.
Related Links	https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/s12864-022-08362-6.pdf
Ending Page	12
Page Count	12
Starting Page	1
File Format	HTM / HTML
ISSN	14712164
DOI	10.1186/s12864-022-08362-6
Journal	BMC Genomics
Issue Number	1
Volume Number	23
Language	English
Publisher	BioMed Central
Publisher Date	2022-02-16
Access Restriction	Open
Subject Keyword	Life Sciences Microarrays Proteomics Animal Genetics and Genomics Microbial Genetics and Genomics Plant Genetics and Genomics NUDT15 Indel Promoter Muscle Primary myoblast Cell proliferation
Content Type	Text
Resource Type	Article
Subject	Biotechnology Genetics
Journal Impact Factor	3.5/2023
5-Year Journal Impact Factor	4.1/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1

Histological, transcriptomic and in vitro analysis reveal an intrinsic activated state of myogenic precursors in hyperplasic muscle of trout

Development of EST-based SNP and InDel markers and their utilization in tetraploid cotton genetic mapping

Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation

MicroRNA expression patterns in post-natal mouse skeletal muscle development

Functional marker development of miR1511-InDel and allelic diversity within the genus Glycine

Development of novel InDel markers and genetic diversity in Chenopodium quinoa through whole-genome re-sequencing

Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18

Minimum InDel pattern analysis of the Zika virus

Genetic effect of an InDel in the promoter region of the NUDT15 and its effect on myoblast proliferation in chickens

Similar Documents

MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1

Histological, transcriptomic and in vitro analysis reveal an intrinsic activated state of myogenic precursors in hyperplasic muscle of trout

Development of EST-based SNP and InDel markers and their utilization in tetraploid cotton genetic mapping

Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation

MicroRNA expression patterns in post-natal mouse skeletal muscle development

Functional marker development of miR1511-InDel and allelic diversity within the genus Glycine

Development of novel InDel markers and genetic diversity in Chenopodium quinoa through whole-genome re-sequencing

Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18

Minimum InDel pattern analysis of the Zika virus

Genetic effect of an InDel in the promoter region of the NUDT15 and its effect on myoblast proliferation in chickens