NDLI: Dynamic expression of Ralstonia solanacearum virulence factors and metabolism-controlling genes during plant infection

Content Provider	Springer Nature : BioMed Central
Author	de Pedro-Jové, R. Puigvert, M. Sebastià, P. Macho, A. P. Monteiro, J. S. Coll, N. S. Setúbal, J. C. Valls, M.
Abstract	Background Ralstonia solanacearum is the causal agent of bacterial wilt, a devastating plant disease responsible for serious economic losses especially on potato, tomato, and other solanaceous plant species in temperate countries. In R. solanacearum, gene expression analysis has been key to unravel many virulence determinants as well as their regulatory networks. However, most of these assays have been performed using either bacteria grown in minimal medium or in planta, after symptom onset, which occurs at late stages of colonization. Thus, little is known about the genetic program that coordinates virulence gene expression and metabolic adaptation along the different stages of plant infection by R. solanacearum. Results We performed an RNA-sequencing analysis of the transcriptome of bacteria recovered from potato apoplast and from the xylem of asymptomatic or wilted potato plants, which correspond to three different conditions (Apoplast, Early and Late xylem). Our results show dynamic expression of metabolism-controlling genes and virulence factors during parasitic growth inside the plant. Flagellar motility genes were especially up-regulated in the apoplast and twitching motility genes showed a more sustained expression in planta regardless of the condition. Xylem-induced genes included virulence genes, such as the type III secretion system (T3SS) and most of its related effectors and nitrogen utilisation genes. The upstream regulators of the T3SS were exclusively up-regulated in the apoplast, preceding the induction of their downstream targets. Finally, a large subset of genes involved in central metabolism was exclusively down-regulated in the xylem at late infection stages. Conclusions This is the first report describing R. solanacearum dynamic transcriptional changes within the plant during infection. Our data define four main genetic programmes that define gene pathogen physiology during plant colonisation. The described expression of virulence genes, which might reflect bacterial states in different infection stages, provides key information on the R. solanacearum potato infection process.
Related Links	https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/s12864-021-07457-w.pdf
Ending Page	18
Page Count	18
Starting Page	1
File Format	HTM / HTML
ISSN	14712164
DOI	10.1186/s12864-021-07457-w
Journal	BMC Genomics
Issue Number	1
Volume Number	22
Language	English
Publisher	BioMed Central
Publisher Date	2021-03-09
Access Restriction	Open
Subject Keyword	Life Sciences Microarrays Proteomics Animal Genetics and Genomics Microbial Genetics and Genomics Plant Genetics and Genomics Ralstonia solanacearum Bacterial wilt RNAseq Virulence factors Dynamic gene expression Metabolism T3SS Effectors Xylem Apoplast
Content Type	Text
Resource Type	Article
Subject	Biotechnology Genetics
Journal Impact Factor	3.5/2023
5-Year Journal Impact Factor	4.1/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Genomic and proteomic evidence supporting the division of the plant pathogen Ralstonia solanacearum into three species

Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors

Transcriptome responses to Ralstonia solanacearum infection in the roots of the wild potato Solanum commersonii

Comparative genomic analysis of Ralstonia solanacearum reveals candidate genes for host specificity

Gene enrichment and co-expression analysis shed light on transcriptional responses to Ralstonia solanacearum in tomato

Plant Assays for Quantifying Ralstonia solanacearum Virulence.

Novel plant inputs influencing Ralstonia solanacearum during infection

Complete genome sequence of the potato pathogen Ralstonia solanacearum UY031

Comparative Genomics of Ralstonia solanacearum Identifies Candidate Genes Associated with Cool Virulence

Dynamic expression of Ralstonia solanacearum virulence factors and metabolism-controlling genes during plant infection

Similar Documents

Genomic and proteomic evidence supporting the division of the plant pathogen Ralstonia solanacearum into three species

Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors

Transcriptome responses to Ralstonia solanacearum infection in the roots of the wild potato Solanum commersonii

Comparative genomic analysis of Ralstonia solanacearum reveals candidate genes for host specificity

Gene enrichment and co-expression analysis shed light on transcriptional responses to Ralstonia solanacearum in tomato

Plant Assays for Quantifying Ralstonia solanacearum Virulence.

Novel plant inputs influencing Ralstonia solanacearum during infection

Complete genome sequence of the potato pathogen Ralstonia solanacearum UY031

Comparative Genomics of Ralstonia solanacearum Identifies Candidate Genes Associated with Cool Virulence

Dynamic expression of Ralstonia solanacearum virulence factors and metabolism-controlling genes during plant infection