NDLI: Genome-wide identification of the 14–3-3 gene family and its participation in floral transition by interacting with TFL1/FT in apple

Content Provider	Springer Nature : BioMed Central
Author	Zuo, Xiya Wang, Shixiang Xiang, Wen Yang, Huiru Tahir, Muhammad Mobeen Zheng, Shangong An, Na Han, Mingyu Zhao, Caiping Zhang, Dong
Abstract	Background Apple (Malus domestica Borkh.) is a popular cultivated fruit crop with high economic value in China. Apple floral transition is an important process but liable to be affected by various environmental factors. The 14–3-3 proteins are involved in regulating diverse biological processes in plants, and some 14–3-3 members play vital roles in flowering. However, little information was available about the 14–3-3 members in apple. Results In the current study, we identified eighteen 14–3-3 gene family members from the apple genome database, designated MdGF14a to MdGF14r. The isoforms possess a conserved core region comprising nine antiparallel α-helices and divergent N and C termini. According to their structural and phylogenetic features, Md14–3-3 proteins could be classified into two major evolutionary branches, the epsilon (ɛ) group and the non-epsilon (non-ɛ) group. Moreover, expression profiles derived from transcriptome data and quantitative real-time reverse transcription PCR analysis showed diverse expression patterns of Md14–3-3 genes in various tissues and in response to different sugars and hormone treatments during the floral transition phase. Four Md14–3-3 isoforms (MdGF14a, MdGF14d, MdGF14i, and MdGF14j) exhibiting prominent transcriptional responses to sugars and hormones were selected for further investigation. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation experiments showed that the four Md14–3-3 proteins interact with key floral integrators, MdTFL1 (TERMINAL FLOWER1) and MdFT (FLOWERING LOCUS T). Subcellular localization of four selected Md14–3-3 proteins demonstrated their localization in both the cytoplasm and nucleus. Conclusion We identified the Md14–3-3 s family in apple comprehensively. Certain Md14–3-3 genes are expressed predominantly during the apple floral transition stage, and may participate in the regulation of flowering through association with flower control genes. Our results provide a preliminary framework for further investigation into the roles of Md14–3-3 s in floral transition.
Related Links	https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/s12864-020-07330-2.pdf
Ending Page	17
Page Count	17
Starting Page	1
File Format	HTM / HTML
ISSN	14712164
DOI	10.1186/s12864-020-07330-2
Journal	BMC Genomics
Issue Number	1
Volume Number	22
Language	English
Publisher	BioMed Central
Publisher Date	2021-01-08
Access Restriction	Open
Subject Keyword	Life Sciences Microarrays Proteomics Animal Genetics and Genomics Microbial Genetics and Genomics Plant Genetics and Genomics Apple 14–3-3 s Genome-wide TFL1 FT Floral transition
Content Type	Text
Resource Type	Article
Subject	Biotechnology Genetics
Journal Impact Factor	3.5/2023
5-Year Journal Impact Factor	4.1/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Genome-wide identification and gene expression analysis of the 14–3-3 gene family in potato (Solanum tuberosum L.)

Genome-wide identification and comparative evolution of 14–3-3 gene family members in five Brassicaceae species

Genome-wide identification, characterization and expression analysis of MATE family genes in apple (Malus × domestica Borkh)

Genome-wide identification and analysis of the GGCT gene family in wheat

Genome-wide identification and molecular evolution of Dof gene family in Camellia oleifera

Genome-wide identification and expression characterization of the GH3 gene family of tea plant (Camellia sinensis)

Genome-wide identification and stress response analysis of cyclophilin gene family in apple (Malus × domestica)

Genome-wide identification and characterization of the TIFY gene family in kiwifruit

Genome-wide identification and expression analysis of the SPL gene family and its response to abiotic stress in barley (Hordeum vulgare L.)

Genome-wide identification of the 14–3-3 gene family and its participation in floral transition by interacting with TFL1/FT in apple

Similar Documents

Genome-wide identification and gene expression analysis of the 14–3-3 gene family in potato (Solanum tuberosum L.)

Genome-wide identification and comparative evolution of 14–3-3 gene family members in five Brassicaceae species

Genome-wide identification, characterization and expression analysis of MATE family genes in apple (Malus × domestica Borkh)

Genome-wide identification and analysis of the GGCT gene family in wheat

Genome-wide identification and molecular evolution of Dof gene family in Camellia oleifera

Genome-wide identification and expression characterization of the GH3 gene family of tea plant (Camellia sinensis)

Genome-wide identification and stress response analysis of cyclophilin gene family in apple (Malus × domestica)

Genome-wide identification and characterization of the TIFY gene family in kiwifruit

Genome-wide identification and expression analysis of the SPL gene family and its response to abiotic stress in barley (Hordeum vulgare L.)

Genome-wide identification of the 14–3-3 gene family and its participation in floral transition by interacting with TFL1/FT in apple