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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Zhao, Zifan Tseng, Yu-Chien Peng, Ze Lopez, Yolanda Chen, Charles Y. Tillman, Barry L. Dang, Phat Wang, Jianping |
| Abstract | Background Spotted wilt, caused by tomato spotted wilt virus (TSWV), has been one of major diseases in cultivated peanut grown in the southeastern United States (US) since 1990. Previously a major quantitative trait locus (QTL) controlling spotted wilt disease resistance was mapped to an interval of 2.55 cM genetic distance corresponding to a physical distance of 14.4 Mb on chromosome A01 of peanut by using a segregating F2 population. The current study focuses on refining this major QTL region and evaluating its contributions in the US peanut mini-core germplasm. Results Two simple sequence repeat (SSR) markers associated with the major QTL were used to genotype F5 individuals, and 25 heterozygous individuals were selected and developed into an F6 segregating population. Based on visual evaluation in the field, a total of 194 susceptible F6 individuals were selected and planted into F7 generation for phenotyping. Nine SSR markers were used to genotype the 194 F6 individuals, and QTL analysis revealed that a confidence interval of 15.2 Mb region had the QTL with 22.8% phenotypic variation explained (PVE). This QTL interval was further genotyped using the Amplicon-seq method. A total of 81 non-redundant single nucleotide polymorphism (SNP) and eight InDel markers were detected. No recombinant was detected among the F6 individuals. Two InDel markers were integrated into the linkage group and helped to refine the confidence interval of this QTL into a 0.8 Mb region. To test the QTL contributes to the resistance variance in US peanut mini-core germplasm, two flanking SSR markers were used to genotype 107 mini-core germplasm accessions. No statistically significant association was observed between the genotype at the QTL region and spotted wilt resistance in the mini-core germplasm, which indicated that the resistance allelic region at this QTL didn’t contribute to the resistance variance in the US peanut mini-core germplasm, thus was a unique resistance source. Conclusion A major QTL related to spotted wilt disease resistance in peanut was refined to a 0.8 Mb region on A01 chromosome, which didn’t relate to spotted wilt disease resistance in the US peanut mini-core germplasm and might be a unique genetic source. |
| Related Links | https://bmcgenomdata.biomedcentral.com/counter/pdf/10.1186/s12863-018-0601-3.pdf |
| Ending Page | 12 |
| Page Count | 12 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 27306844 |
| DOI | 10.1186/s12863-018-0601-3 |
| Journal | BMC Genomic Data |
| Issue Number | 1 |
| Volume Number | 19 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2018-03-23 |
| Access Restriction | Open |
| Subject Keyword | Life Sciences Animal Genetics and Genomics Microbial Genetics and Genomics Plant Genetics and Genomics Genetics and Population Dynamics Cultivated peanut Tomato spotted wilt virus (TSWV) Spotted wilt Simple sequence repeat (SSR) Single nucleotide polymorphism (SNP) Spotted wilt disease Quantitative trait loci (QTL) US peanut mini-core germplasm |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health Informatics Genetics |
| Journal Impact Factor | 1.9/2023 |
| 5-Year Journal Impact Factor | 1.9/2023 |
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