NDLI: Expression of proposed methionine transporters along the gastrointestinal tract of pigs and their regulation by dietary methionine sources

Please wait, while we are loading the content...

Effect of AMY1 copy number variation and various doses of starch intake on glucose homeostasis: data from a cross-sectional observational study and a crossover meal study

Genetic predisposition to impaired metabolism of the branched chain amino acids, dietary intakes, and risk of type 2 diabetes

Mendelian randomization to evaluate the effect of plasma vitamin C levels on the risk of Alzheimer’s disease

Correction to: Variation in the vitamin D receptor gene, plasma 25-hydroxyvitamin D, and risk of premenstrual symptoms

Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency

Vegetarian diet duration’s influence on women’s gut environment

Variation in the vitamin D receptor gene, plasma 25-hydroxyvitamin D, and risk of premenstrual symptoms

Expression of proposed methionine transporters along the gastrointestinal tract of pigs and their regulation by dietary methionine sources

Expression of the circular RNAs in astaxanthin promotes cholesterol efflux from THP-1 cells based on RNA-seq

Transcriptomic changes in peripheral blood mononuclear cells with weight loss: systematic literature review and primary data synthesis

Correction to: TCONS_00230836 silencing restores stearic acid-induced β cell dysfunction through alleviating endoplasmic reticulum stress rather than apoptosis

The association of circulating miR-191 and miR-375 expression levels with markers of insulin resistance in overweight children: an exploratory analysis of the I.Family Study

Serum iron status and the risk of breast cancer in the European population: a two-sample Mendelian randomisation study

TCONS_00230836 silencing restores stearic acid-induced β cell dysfunction through alleviating endoplasmic reticulum stress rather than apoptosis

An 8-week freeze-dried blueberry supplement impacts immune-related pathways: a randomized, double-blind placebo-controlled trial

A systematic review to identify biomarkers of intake for fermented food products

Association of vitamin D pathway genes polymorphisms with pulmonary tuberculosis susceptibility in a Chinese population

Interaction between dietary branched-chain amino acids and genetic risk score on the risk of type 2 diabetes in Chinese

Consumer acceptance of genetic-based personalized nutrition in Hungary

Will guidelines on alcohol consumption be personalized by a genetic approach?

Maternal folic acid impacts DNA methylation profile in male rat offspring implicated in neurodevelopment and learning/memory abilities

Expression of proposed methionine transporters along the gastrointestinal tract of pigs and their regulation by dietary methionine sources

Content Provider	Springer Nature : BioMed Central
Author	Romanet, Stella Aschenbach, Jörg R. Pieper, Robert Zentek, Jürgen Htoo, John K. Whelan, Rose A. Mastrototaro, Lucia
Abstract	Background Given the key role of methionine (Met) in biological processes like protein translation, methylation, and antioxidant defense, inadequate Met supply can limit performance. This study investigated the effect of different dietary Met sources on the expression profile of various Met transporters along the gastrointestinal tract (GIT) of pigs. Methods A total of 27 pigs received a diet supplemented with 0.21% DL-Met, 0.21% L-Met, or 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA). Changes in mRNA expression of B0AT1, ATB0,+, rBAT, ASCT2, IMINO, LAT4, y+LAT1, LAT2, and SNAT2 were evaluated in the oral mucosa, cardia, fundus, pylorus, duodenum, proximal jejunum, middle jejunum, ileum, cecum, proximal colon, and distal colon, complemented by protein expression analysis of B0AT1, ASCT2, LAT2, and LAT4. Results Expression of all investigated transcripts differed significantly along the GIT. B0AT1, rBAT, y+LAT1, LAT2, and LAT4 showed strongest mRNA expression in small intestinal segments. ASCT2, IMINO, and SNAT2 were similarly expressed along the small and large intestines but expression differed in the oral mucosa and stomach. ATB0,+ showed highest mRNA expression in large intestinal tissues, cardia, and pylorus. In pigs fed DL-Met, mRNA expression of ASCT2 was higher than in pigs fed DL-HMTBA in small intestinal tissues and mRNA expression of IMINO was lower than in pigs fed L-Met in large intestinal tissues. Dietary DL-HMTBA induced a stronger mRNA expression of basolateral uptake systems either in the small (LAT2) or large (y+LAT1) intestine. Protein expression of B0AT1 was higher in the middle jejunum and ileum in pigs fed DL-Met when compared with the other Met supplements. LAT4 expression was higher in pigs fed DL-HMTBA when compared with DL-Met (small intestine) and L-Met (small intestine, oral mucosa, and stomach). Conclusion A high expression of several Met transporters in small intestinal segments underlines the primary role of these segments in amino acid absorption; however, some Met transporters show high transcript and protein levels also in large intestine, oral mucosa, and stomach. A diet containing DL-Met has potential to increase apical Met transport in the small intestine, whereas a diet containing DL-HMTBA has potential to increase basolateral Met transport in the small intestine and, partly, other gastrointestinal tissues.
Related Links	https://genesandnutrition.biomedcentral.com/counter/pdf/10.1186/s12263-021-00694-4.pdf
Ending Page	15
Page Count	15
Starting Page	1
File Format	HTM / HTML
ISSN	18653499
DOI	10.1186/s12263-021-00694-4
Journal	Genes & Nutrition
Issue Number	1
Volume Number	16
Language	English
Publisher	BioMed Central
Publisher Date	2021-09-06
Access Restriction	Open
Subject Keyword	Human Genetics Clinical Nutrition Gene Function Biomedicine Longitudinal heterogeneity of gene expression Intestine Methionine transport qRT-PCR Stomach Western blot
Content Type	Text
Resource Type	Article
Subject	Endocrinology, Diabetes and Metabolism Genetics
Journal Impact Factor	3.3/2023
5-Year Journal Impact Factor	3.8/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The Expression of Zinc Transporters Changed in the Intestine of Weaned Pigs Exposed to Zinc Chitosan Chelate

Oral administration of Lactobacillus plantarum 299v modulates gene expression in the ileum of pigs: prediction of crosstalk between intestinal immune cells and sub-mucosal adipocytes

Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa

The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines

Differences in the expression pattern of HCN isoforms among mammalian tissues: sources and implications

Proposed guidelines to evaluate scientific validity and evidence for genotype-based dietary advice

Reduced expression of folate transporters in kidney of a rat model of folate oversupplementation

Somatostatin and the gastrointestinal tract.

Ancestors’ dietary patterns and environments could drive positive selection in genes involved in micronutrient metabolism—the case of cofactor transporters

Expression of proposed methionine transporters along the gastrointestinal tract of pigs and their regulation by dietary methionine sources

Similar Documents

The Expression of Zinc Transporters Changed in the Intestine of Weaned Pigs Exposed to Zinc Chitosan Chelate

Oral administration of Lactobacillus plantarum 299v modulates gene expression in the ileum of pigs: prediction of crosstalk between intestinal immune cells and sub-mucosal adipocytes

Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa

The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines

Differences in the expression pattern of HCN isoforms among mammalian tissues: sources and implications

Proposed guidelines to evaluate scientific validity and evidence for genotype-based dietary advice

Reduced expression of folate transporters in kidney of a rat model of folate oversupplementation

Somatostatin and the gastrointestinal tract.

Ancestors’ dietary patterns and environments could drive positive selection in genes involved in micronutrient metabolism—the case of cofactor transporters

Expression of proposed methionine transporters along the gastrointestinal tract of pigs and their regulation by dietary methionine sources