NDLI: Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients

Content Provider	Springer Nature : BioMed Central
Author	Turrel-Davin, Fanny Guignant, Caroline Lepape, Alain Mougin, Bruno Monneret, Guillaume Venet, Fabienne
Abstract	Introduction Lymphocyte apoptosis has been suggested to play a central role in sepsis pathophysiology, and studies in animal models demonstrated that blocking this pathway improves outcome. However, no routine biomarkers of apoptosis are so far available in patients. Thus, the aim of our study was to assess the different biomarkers of apoptosis putatively usable on a routine basis in septic shock. Methods Thirteen septic shock patients (sampled twice between days 1 to 2 and days 3 to 5 after diagnosis of shock) and 15 sex-matched and age-matched healthy controls were prospectively enrolled. Apoptosis was measured in lymphocyte subpopulations using flow cytometry (Annexin-V binding, activated caspase-3 and Bcl-2 expressions). Representative pro-apoptotic and anti-apoptotic gene expressions were assessed by quantitative reverse-transcription PCR. Monocyte HLA-DR expression and lymphocyte subpopulation cell counts were measured as markers of sepsis-induced immune dysfunctions. To test for statistical significance, the Mann-Whitney U test was used with correction by the number of tests performed. Results Flow cytometric measurements of apoptosis in septic shock patients showed an increased Annexin-V binding on CD4+ T cells and an increased active caspase-3 expression on B cells only at days 3 to 5 (sixfold change and twofold change, respectively). Gene expression analysis showed an increased BCL-XL mRNA and an upregulation of the pro-apoptotic genes BID and FAS in septic shock patients (10-fold change and fivefold change, respectively) compared with healthy controls. Conclusions The present study highlights the difficulties encountered in monitoring apoptosis on a routine basis in septic patients, whereas in the same sampling conditions and on the same patients, HLA-DR expression and lymphocyte subpopulation cell counts showed characteristics described in the literature. However, pro-apoptotic genes BID and FAS appear to constitute promising apoptosis markers in our hands.
Related Links	https://ccforum.biomedcentral.com/counter/pdf/10.1186/cc9181.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
ISSN	13648535
DOI	10.1186/cc9181
Journal	Critical Care
Issue Number	4
Volume Number	14
Language	English
Publisher	BioMed Central
Publisher Date	2010-07-13
Access Restriction	Open
Subject Keyword	Intensive Critical Care Medicine Emergency Medicine Septic Shock Septic Patient Septic Shock Patient Routine Basis Lymphocyte Apoptosis
Content Type	Text
Resource Type	Article
Subject	Critical Care and Intensive Care Medicine
Journal Impact Factor	8.8/2023
5-Year Journal Impact Factor	10.4/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

RESEARCH Open Access Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients

Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients

Early alterations of B cells in patients with septic shock

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients

Altered T-cell repertoire diversity in septic shock patients

Leukocyte phenotyping to stratify septic shock patients

HLA-DR expression on monocytes and the T-cell subset in septic patients

CD4+ T-lymphocyte alterations in trauma patients

Association between apoptosis and mortality in severe septic patients

Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients

Similar Documents

RESEARCH Open Access Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients

Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients

Early alterations of B cells in patients with septic shock

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients

Altered T-cell repertoire diversity in septic shock patients

Leukocyte phenotyping to stratify septic shock patients

HLA-DR expression on monocytes and the T-cell subset in septic patients

CD4+ T-lymphocyte alterations in trauma patients

Association between apoptosis and mortality in severe septic patients

Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients