| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Wang, Fang Xu, Lingxiao Feng, Xiaoke Guo, Dunming Tan, Wenfeng Zhang, Miaojia |
| Abstract | Introduction The immunoregulatory function of interleukin (IL)-29 has recently been recognized. However, little is known about the involvement of IL-29 in the pathogenesis of rheumatoid arthritis (RA). This study aimed to examine the expression profiles of IL-29 in blood, synovial fluid (SF) and synovium in RA patients and investigate the effect of IL-29 on cytokines production in RA synovial fibroblasts. Methods The transcript levels of IL-29 and its specific receptor IL-28Rα in peripheral blood mononuclear cells (PBMC) and synovium were determined by real-time reverse transcription-polymerase chain reaction (real-time PCR). The concentrations of IL-29 in serum and synovial fluid (SF) were quantified by enzyme-linked immunoassay (ELISA), and the correlation of serum IL-29 levels with disease activity in RA patients was investigated. Furthermore, the expression of IL-29 in RA synovium was examined by immunohistochemistry and double immunofluorescence analysis. Finally, the expression of IL-6, IL-8, IL-10, IL-17 and matrix metalloproteinase-3 (MMP-3) in synovial fibroblasts upon IL-29 stimulation was determined by real-time PCR. Results IL-29 and IL-28Rα mRNA expression in PBMC was significantly increased in patients with RA compared with healthy controls (HC). The serum levels of circulating IL-29 were higher in RA than those in HC. Increased IL-29 levels were detected in RA SF when compared with osteoarthritis (OA) SF. However, serum IL-29 levels showed no significant correlation with RA disease activity. IL-29 was mostly expressed in the lining region of RA synovium. Moreover, IL-29 was expressed predominately in synovial macrophages and fibroblasts. RA synovial fibroblasts exposed to IL-29 specifically upregulated IL-6, -8 and MMP-3 but downregulated IL-10. Conclusions The findings in the present study indicate, for the first time, that IL-29 is dysregulated in patients with RA, which may contribute to the RA pathogenesis via inducing the production of proinflammatory cytokines, chemokines or matrix metalloproteinases in synovial fibroblasts. |
| Related Links | https://arthritis-research.biomedcentral.com/counter/pdf/10.1186/ar4067.pdf |
| Ending Page | 10 |
| Page Count | 10 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14786362 |
| DOI | 10.1186/ar4067 |
| Journal | Arthritis Research & Therapy |
| Issue Number | 5 |
| Volume Number | 14 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2012-10-19 |
| Access Restriction | Open |
| Subject Keyword | Rheumatology Orthopedics Rheumatoid Arthritis Rheumatoid Arthritis Patient Synovial Fluid Synovial Tissue Synovial Fibroblast |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology Rheumatology |
| Journal Impact Factor | 4.4/2023 |
| 5-Year Journal Impact Factor | 4.9/2023 |
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