NDLI: The distribution pattern of critically short telomeres in human osteoarthritic knees

Content Provider	Springer Nature : BioMed Central
Author	Harbo, Maria Bendix, Laila Bay-Jensen, Anne-Christine Graakjaer, Jesper Søe, Kent Andersen, Thomas L Kjaersgaard-Andersen, Per Koelvraa, Steen Delaisse, Jean-Marie
Abstract	Introduction Telomere shortening is associated with a number of common age-related diseases. A role of telomere shortening in osteoarthritis (OA) has been suggested, mainly based on the assessment of mean telomere length in ex vivo expanded chondrocytes. We addressed this role directly in vivo by using a newly developed assay, which measures specifically the load of ultra-short single telomeres (below 1,500 base pairs), that is, the telomere subpopulation believed to promote cellular senescence. Methods Samples were obtained from human OA knees at two distances from the central lesion site. Each sample was split into three: one was used for quantification of ultra-short single telomeres through the Universal single telomere length assay (STELA), one for histological Mankin grading of OA, and one for mean telomere length measurement through quantitative fluorescence in situ hybridization (Q-FISH) as well as for assessment of senescence through quantification of senescence-associated heterochromatin foci (SAHF). Results The load of ultra-short telomeres as well as mean telomere length was significantly associated with proximity to lesions, OA severity, and senescence level. The degree of significance was higher when assessed through load of ultra-short telomeres per cell compared with mean telomere length. Conclusions These in vivo data, especially the quantification of ultra-short telomeres, stress a role of telomere shortening in human OA.
Related Links	https://arthritis-research.biomedcentral.com/counter/pdf/10.1186/ar3687.pdf
Ending Page	9
Page Count	9
Starting Page	1
File Format	HTM / HTML
ISSN	14786362
DOI	10.1186/ar3687
Journal	Arthritis Research & Therapy
Issue Number	1
Volume Number	14
Language	English
Publisher	BioMed Central
Publisher Date	2012-01-18
Access Restriction	Open
Subject Keyword	Rheumatology Orthopedics Telomere Length Short Telomere Peptide Nucleic Acid Probe Mankin Score Telomere Length Measurement
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology Rheumatology
Journal Impact Factor	4.4/2023
5-Year Journal Impact Factor	4.9/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The distribution pattern of critically short telomeres in human osteoarthritic knees

Regenerative potential of human muscle stem cells in chronic inflammation

Proliferation and differentiation potential of chondrocytes from osteoarthritic patients

Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

Critically short telomeres and toxicity of chemotherapy in early breast cancer.

Oxidative stress induces chondrocyte telomere instability and chondrocyte dysfunctions in osteoarthritis

Molecular response to cartilage injury

Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage

UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis

The distribution pattern of critically short telomeres in human osteoarthritic knees

Similar Documents

The distribution pattern of critically short telomeres in human osteoarthritic knees

Regenerative potential of human muscle stem cells in chronic inflammation

Proliferation and differentiation potential of chondrocytes from osteoarthritic patients

Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

Critically short telomeres and toxicity of chemotherapy in early breast cancer.

Oxidative stress induces chondrocyte telomere instability and chondrocyte dysfunctions in osteoarthritis

Molecular response to cartilage injury

Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage

UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis

The distribution pattern of critically short telomeres in human osteoarthritic knees