NDLI: Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production

Content Provider	Springer Nature : BioMed Central
Author	Shen, Hui Poovaiah, Charleson R Ziebell, Angela Tschaplinski, Timothy J Pattathil, Sivakumar Gjersing, Erica Engle, Nancy L Katahira, Rui Pu, Yunqiao Sykes, Robert Chen, Fang Ragauskas, Arthur J Mielenz, Jonathan R Hahn, Michael G Davis, Mark Stewart, C Neal Dixon, Richard A
Abstract	Background Lignocellulosic biomass is one of the most promising renewable and clean energy resources to reduce greenhouse gas emissions and dependence on fossil fuels. However, the resistance to accessibility of sugars embedded in plant cell walls (so-called recalcitrance) is a major barrier to economically viable cellulosic ethanol production. A recent report from the US National Academy of Sciences indicated that, “absent technological breakthroughs”, it was unlikely that the US would meet the congressionally mandated renewable fuel standard of 35 billion gallons of ethanol-equivalent biofuels plus 1 billion gallons of biodiesel by 2022. We here describe the properties of switchgrass (Panicum virgatum) biomass that has been genetically engineered to increase the cellulosic ethanol yield by more than 2-fold. Results We have increased the cellulosic ethanol yield from switchgrass by 2.6-fold through overexpression of the transcription factor PvMYB4. This strategy reduces carbon deposition into lignin and phenolic fermentation inhibitors while maintaining the availability of potentially fermentable soluble sugars and pectic polysaccharides. Detailed biomass characterization analyses revealed that the levels and nature of phenolic acids embedded in the cell-wall, the lignin content and polymer size, lignin internal linkage levels, linkages between lignin and xylans/pectins, and levels of wall-bound fucose are all altered in PvMYB4-OX lines. Genetically engineered PvMYB4-OX switchgrass therefore provides a novel system for further understanding cell wall recalcitrance. Conclusions Our results have demonstrated that overexpression of PvMYB4, a general transcriptional repressor of the phenylpropanoid/lignin biosynthesis pathway, can lead to very high yield ethanol production through dramatic reduction of recalcitrance. MYB4-OX switchgrass is an excellent model system for understanding recalcitrance, and provides new germplasm for developing switchgrass cultivars as biomass feedstocks for biofuel production.
Related Links	https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/1754-6834-6-71.pdf
Ending Page	15
Page Count	15
Starting Page	1
File Format	HTM / HTML
ISSN	27313654
DOI	10.1186/1754-6834-6-71
Journal	Biotechnology for Biofuels and Bioproducts
Issue Number	1
Volume Number	6
Language	English
Publisher	BioMed Central
Publisher Date	2013-05-07
Access Restriction	Open
Subject Keyword	Biotechnology Plant Breeding Environmental Engineering Renewable and Green Energy Microbiology Switchgrass Bioenergy Biofuel Feedstock Cellulosic ethanol PvMYB4 Transcription factor Cell wall Recalcitrance Lignin Hemicellulose Pectin Plant Breeding/Biotechnology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Energy Management, Monitoring, Policy and Law Renewable Energy, Sustainability and the Environment Applied Microbiology and Biotechnology Biotechnology
Journal Impact Factor	6.1/2023
5-Year Journal Impact Factor	6/2023

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