NDLI: Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides

Content Provider	Springer Nature : BioMed Central
Author	Gao, Dahai Uppugundla, Nirmal Chundawat, Shishir PS Yu, Xiurong Hermanson, Spencer Gowda, Krishne Brumm, Phillip Mead, David Balan, Venkatesh Dale, Bruce E
Abstract	Background High enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the enzyme cocktail for specific types of pretreated biomass allows for a significant reduction in enzyme loading without sacrificing hydrolysis yield. This is especially important for alkaline pretreatments such as Ammonia fiber expansion (AFEX) pretreated corn stover. Hence, a diverse set of hemicellulases supplemented along with cellulases is necessary for high recovery of monosaccharides. Results The core fungal cellulases in the optimal cocktail include cellobiohydrolase I [CBH I; glycoside hydrolase (GH) family 7A], cellobiohydrolase II (CBH II; GH family 6A), endoglucanase I (EG I; GH family 7B) and β-glucosidase (βG; GH family 3). Hemicellulases tested along with the core cellulases include xylanases (LX1, GH family 10; LX2, GH family 10; LX3, GH family 10; LX4, GH family 11; LX5, GH family 10; LX6, GH family 10), β-xylosidase (LβX; GH family 52), α-arabinofuranosidase (LArb, GH family 51) and α-glucuronidase (LαGl, GH family 67) that were cloned, expressed and/or purified from different bacterial sources. Different combinations of these enzymes were tested using a high-throughput microplate based 24 h hydrolysis assay. Both family 10 (LX3) and family 11 (LX4) xylanases were found to most efficiently hydrolyze AFEX pretreated corn stover in a synergistic manner. The optimal mass ratio of xylanases (LX3 and LX4) to cellulases (CBH I, CBH II and EG I) is 25:75. LβX (0.6 mg/g glucan) is crucial to obtaining monomeric xylose (54% xylose yield), while LArb (0.6 mg/g glucan) and LαGl (0.8 mg/g glucan) can both further increase xylose yield by an additional 20%. Compared with Accellerase 1000, a purified cocktail of cellulases supplemented with accessory hemicellulases will not only increase both glucose and xylose yields but will also decrease the total enzyme loading needed for equivalent yields. Conclusions A diverse set of accessory hemicellulases was found necessary to enhance the synergistic action of cellulases hydrolysing AFEX pretreated corn stover. High glucose (around 80%) and xylose (around 70%) yields were achieved with a moderate enzyme loading (~20 mg protein/g glucan) using an in-house developed cocktail compared to commercial enzymes.
Related Links	https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/1754-6834-4-5.pdf
Ending Page	11
Page Count	11
Starting Page	1
File Format	HTM / HTML
ISSN	27313654
DOI	10.1186/1754-6834-4-5
Journal	Biotechnology for Biofuels and Bioproducts
Issue Number	1
Volume Number	4
Language	English
Publisher	BioMed Central
Publisher Date	2011-02-22
Access Restriction	Open
Subject Keyword	Biotechnology Plant Breeding Environmental Engineering Renewable and Green Energy Microbiology Xylose Cellulase Glucan Corn Stover Glucose Yield Plant Breeding/Biotechnology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Energy Management, Monitoring, Policy and Law Renewable Energy, Sustainability and the Environment Applied Microbiology and Biotechnology Biotechnology
Journal Impact Factor	6.1/2023
5-Year Journal Impact Factor	6/2023

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Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides

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Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides

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