NDLI: Method to grow Actinobacillus pleuropneumoniaebiofilm on a biotic surface

Content Provider	Springer Nature : BioMed Central
Author	Tremblay, Yannick DN Lévesque, Cynthia Segers, Ruud PAM Jacques, Mario
Abstract	Background Actinobacillus pleuropneumoniae is a Gram-negative bacterium and a member of the Pasteurellaceae family. This bacterium is the causative agent of porcine pleuropneumonia, which is a highly contagious respiratory disease causing important economical losses to the worldwide pig industry. It has been shown that A. pleuropneumoniae can form biofilms on abiotic surfaces (plastic and glass). Although in vitro models are extremely useful to gain information on biofilm formation, these models may not be representative of the conditions found at the mucosal surface of the host, which is the natural niche of A. pleuropneumoniae. Results In this paper, we describe a method to grow A. pleuropneumoniae biofilms on the SJPL cell line, which represents a biotic surface. A non-hemolytic, non-cytotoxic mutant of A. pleuropneumoniae was used in our assays and this allowed the SJPL cell monolayers to be exposed to A. pleuropneumoniae for longer periods. This resulted in the formation of biofilms on the cell monolayer after incubations of 24 and 48 h. The biofilms can be stained with fluorescent probes, such as a lectin against the polymer of N-acetyl-D-glucosamine present in the biofilm matrix, and easily observed by confocal laser scanning microscopy. Conclusions This is the first protocol that describes the formation of an A. pleuropneumoniae biofilm on a biotic surface. The advantage of this protocol is that it can be used to study biofilm formation in a context of host-pathogen interactions. The protocol could also be adapted to evaluate biofilm inhibitors or the efficacy of antibiotics in the presence of biofilms.
Related Links	https://bmcvetres.biomedcentral.com/counter/pdf/10.1186/1746-6148-9-213.pdf
Ending Page	8
Page Count	8
Starting Page	1
File Format	HTM / HTML
ISSN	17466148
DOI	10.1186/1746-6148-9-213
Journal	BMC Veterinary Research
Issue Number	1
Volume Number	9
Language	English
Publisher	BioMed Central
Publisher Date	2013-10-20
Access Restriction	Open
Subject Keyword	Veterinary Medicine Veterinary Science Zoology Transgenics Actinobacillus pleuropneumoniae Biofilm Biotic surface SJPL cell line Confocal laser scanning microscopy Host-pathogen interaction Veterinary Medicine/Veterinary Science
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine
Journal Impact Factor	2.3/2023
5-Year Journal Impact Factor	2.6/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Method to grow Actinobacillus pleuropneumoniae biofilm on a biotic surface

Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication

N-terminus of flagellin enhances vaccine efficacy against Actinobacillus pleuropneumoniae

Host-pathogen interplay at primary infection sites in pigs challenged with Actinobacillus pleuropneumoniae

Optimal regimens based on PK/PD cutoff evaluation of ceftiofur against Actinobacillus pleuropneumoniae in swine

Host-pathogen interactions of actinobacillus pleuropneumoniae with (2008).

Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Naringin attenuates Actinobacillus pleuropneumoniae-induced acute lung injury via MAPK/NF-κB and Keap1/Nrf2/HO-1 pathway

Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae

Method to grow Actinobacillus pleuropneumoniaebiofilm on a biotic surface

Similar Documents

Method to grow Actinobacillus pleuropneumoniae biofilm on a biotic surface

Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication

N-terminus of flagellin enhances vaccine efficacy against Actinobacillus pleuropneumoniae

Host-pathogen interplay at primary infection sites in pigs challenged with Actinobacillus pleuropneumoniae

Optimal regimens based on PK/PD cutoff evaluation of ceftiofur against Actinobacillus pleuropneumoniae in swine

Host-pathogen interactions of actinobacillus pleuropneumoniae with (2008).

Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Naringin attenuates Actinobacillus pleuropneumoniae-induced acute lung injury via MAPK/NF-κB and Keap1/Nrf2/HO-1 pathway

Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae

Method to grow Actinobacillus pleuropneumoniaebiofilm on a biotic surface