NDLI: Design of a microscopic electrical impedance tomography system for 3D continuous non-destructive monitoring of tissue culture

Content Provider	Springer Nature : BioMed Central
Author	Lee, Eun Jung Wi, Hun McEwan, Alistair Lee Farooq, Adnan Sohal, Harsh Woo, Eung Je Seo, Jin Keun Oh, Tong In
Abstract	Background Non-destructive continuous monitoring of regenerative tissue is required throughout the entire period of in vitro tissue culture. Microscopic electrical impedance tomography (micro-EIT) has the potential to monitor the physiological state of tissues by forming three-dimensional images of impedance changes in a non-destructive and label-free manner. We developed a new micro-EIT system and report on simulation and experimental results of its macroscopic model. Methods We propose a new micro-EIT system design using a cuboid sample container with separate current-driving and voltage sensing electrodes. The top is open for sample manipulations. We used nine gold-coated solid electrodes on each of two opposing sides of the container to produce multiple linearly independent internal current density distributions. The 360 voltage sensing electrodes were placed on the other sides and base to measure induced voltages. Instead of using an inverse solver with the least squares method, we used a projected image reconstruction algorithm based on a logarithm formulation to produce projected images. We intended to improve the quality and spatial resolution of the images by increasing the number of voltage measurements subject to a few injected current patterns. We evaluated the performance of the micro-EIT system with a macroscopic physical phantom. Results The signal-to-noise ratio of the developed micro-EIT system was 66 dB. Crosstalk was in the range of -110.8 to -90.04 dB. Three-dimensional images with consistent quality were reconstructed from physical phantom data over the entire domain. From numerical and experimental results, we estimate that at least 20 × 40 electrodes with 120 μ m spacing are required to monitor the complex shape of ingrowth neotissue inside a scaffold with 300 μ m pore. Conclusion The experimental results showed that the new micro-EIT system with a reduced set of injection current patterns and a large number of voltage sensing electrodes can be potentially used for tissue culture monitoring. Numerical simulations demonstrated that the spatial resolution could be improved to the scale required for tissue culture monitoring. Future challenges include manufacturing a bioreactor-compatible container with a dense array of electrodes and a larger number of measurement channels that are sensitive to the reduced voltage gradients expected at a smaller scale.
Related Links	https://biomedical-engineering-online.biomedcentral.com/counter/pdf/10.1186/1475-925X-13-142.pdf
Ending Page	15
Page Count	15
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/1475-925X-13-142
Journal	BioMedical Engineering OnLine
Issue Number	1
Volume Number	13
Language	English
Publisher	BioMed Central
Publisher Date	2014-10-06
Access Restriction	Open
Subject Keyword	Biomedical Engineering and Bioengineering Biomaterials Biotechnology Biomedical Engineering Non-destructive monitoring Label-free Electrical impedance tomography Three-dimensional impedance image Tissue culture monitoring Projected image reconstruction algorithm Biomedical Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Biomaterials Radiological and Ultrasound Technology Biomedical Engineering Radiology, Nuclear Medicine and Imaging
Journal Impact Factor	2.9/2023
5-Year Journal Impact Factor	3.5/2023

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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