NDLI: Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense

Content Provider	Springer Nature : BioMed Central
Author	Castillo, Almudena Budak, Hikmet Varshney, Rajeev K Dorado, Gabriel Graner, Andreas Hernandez, Pilar
Abstract	Background Hordeum chilense, a native South American diploid wild barley, is a potential source of useful genes for cereal breeding. The use of this wild species to increase genetic variation in cereals will be greatly facilitated by marker-assisted selection. Different economically feasible approaches have been undertaken for this wild species with limited direct agricultural use in a search for suitable and cost-effective markers. The availability of Expressed Sequence Tags (EST) derived microsatellites or simple sequence repeat (SSR) markers, commonly called as EST-SSRs, for barley (Hordeum vulgare) represents a promising source to increase the number of genetic markers available for the H. chilense genome. Results All of the 82 barley EST-derived SSR primer pairs tested for transferability to H. chilense amplified products of correct size from this species. Of these 82 barley EST-SSRs, 21 (26%) showed polymorphism among H. chilense lines. Identified polymorphic markers were used to test the transferability and polymorphism in other Poaceae family species with the aim of establishing H. chilense phylogenetic relationships. Triticum aestivum-H. chilense addition lines allowed us to determine the chromosomal localizations of EST-SSR markers and confirm conservation of the linkage group. Conclusion From the present study a set of 21 polymorphic EST-SSR markers have been identified to be useful for diversity analysis of H. chilense, related wild barleys like H. murinum, and for wheat marker-assisted introgression breeding. Across-genera transferability of the barley EST-SSR markers has allowed phylogenetic inference within the Triticeae complex.
Related Links	https://bmcplantbiol.biomedcentral.com/counter/pdf/10.1186/1471-2229-8-97.pdf
Ending Page	9
Page Count	9
Starting Page	1
File Format	HTM / HTML
ISSN	14712229
DOI	10.1186/1471-2229-8-97
Journal	BMC Plant Biology
Issue Number	1
Volume Number	8
Language	English
Publisher	BioMed Central
Publisher Date	2008-09-28
Access Restriction	Open
Subject Keyword	Plant Sciences Agriculture Tree Biology Simple Sequence Repeat Marker Wild Barley Genomic SSRs Triticeae Species Genomic Simple Sequence Repeat Marker
Content Type	Text
Resource Type	Article
Subject	Plant Science
Journal Impact Factor	4.3/2023
5-Year Journal Impact Factor	5.2/2023

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